In Vitro Maintenance of Clonorchis sinensis Adult Worms

Md. Hafiz Uddin; Shunyu Li; Young Mee Bae; Min-Ho Choi; Sung-Tae Hong

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자료유형: 학술저널

저자정보: Md. Hafiz Uddin (Seoul National University College of Medicine) Shunyu Li (Seoul National University College of Medicine) Young Mee Bae (Seoul National University College of Medicine) Min-Ho Choi (Seoul National University College of Medicine) Sung-Tae Hong (Seoul National University College of Medicine)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.50 No.4

발행연도: 2012.12

수록면: 309 - 315 (7page)

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Clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in East Asia. The present study investigated survival longevity of C. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. The worms were maintained in 0.85% NaCl, 1×PBS, 1×Locke’s solution, RPMI-1640, DMEM, and IMDM media, and in 1×Locke’s solution with different supplements. All of the worms died within 3 and 7 days in 0.85% NaCl and 1×PBS, respectively, but survived up to 57 days in 1×Locke’s solution. The worms lived for 106 days in DMEM, and 114 days in both RPMI-1640 and IMDM media. The survival rate in RPMI-1640 medium was the highest (50%) compared to that in DMEM (20±10%) and in IMDM (33.3±25.2%) after 3 months. The 1×Locke’s solution with 0.005% bovine bile supplement showed increased duration of maximum survival from 42 days to 70 days. Higher concentration of bile supplements than 0.005% or addition of glucose were disadvantageous for the worm survival. The worms died rapidly in solutions containing L-aspartic acid, L-glutamic acid, and adenine compared to L-arginine, L-serine, and L-tryptophan. In conclusion, the 1×Locke’s solution best supports the worms alive among inorganic solutions for 57 days, and the RPMI-1640 medium maintains living C. sinensis adults better and longer up to 114 days in vitro than other media.

#Clonorchis sinensis #survival #solution #media #in vitro maintenance

참고문헌 (29)

참고문헌 신청

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