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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.12
- 수록면
- 365 - 369 (5page)
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초록· 키워드
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Acanthamoeba spp. are single-celled protozoan organisms that are widely distributed in the environment. In this study, to understand functional roles of a mannose-binding protein (MBP), Acanthamoeba castellanii was treated with methyl-alpha-D-mannopyranoside (mannose), and adhesion and cytotoxicity of the amoeba were analyzed. In addition, to understand the association of MBP for amoeba phagocytosis, phagocytosis assay was analyzed using non-pathogenic bacterium, Escherichia coli K12. Amoebae treated with mannose for 20 cycles exhibited larger vacuoles occupying the most area of the amoebic cytoplasm in comparison with the control group amoebae and glucose-treated amoebae. Mannose-selected amoebae exhibited lower levels of binding to Chinese hamster ovary (CHO) cells. Exogenous mannose inhibited >50% inhibition of amoebae (control group) binding to CHO cells. Moreover, exogenous mannose inhibited amoebae (i.e., man-treated) binding to CHO cells by <15%. Mannose-selected amoebae exhibited significantly decreased cytotoxicity to CHO cells compared with the control group amoebae, 25.1% vs 92.1%. In phagocytic assay, mannose-selected amoebae exhibited significant decreases in bacterial uptake in comparison with the control group, 0.019% vs 0.03% (P<0.05). Taken together, it is suggested that mannose-selected A. castellanii trophozoites should be severely damaged and do not well interact with a target cell via a lectin of MBP.
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참고문헌 (13)
참고문헌 신청
Khan NA / 2006 / Acanthamoeba: biology and increasing importance in human health / FEMS Microbiol Rev 30:564~595
Marciano-Cabral F / 2003 / Acanthamoeba spp. as agents of disease in humans / Clin Microbiol Rev 16:273~307
Siddiqui R / 2012 / Biology and pathogenesis of Acanthamoeba / Parasit Vectors 5:6
Visvesvara GS / 2007 / Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea / FEMS Immunol Med Microbiol 50:1~26
Garate M / 2006 / In vitro pathogenicity of Acanthamoeba is associated with the expression of the mannose-binding protein / Invest Ophthalmol Vis Sci 47:1056~1062
Marciano-Cabral F / 2003 / Acanthamoeba spp. as agents of disease in humans / Clin Microbiol Rev 16:273~307
Siddiqui R / 2012 / Biology and pathogenesis of Acanthamoeba / Parasit Vectors 5:6
Visvesvara GS / 2007 / Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea / FEMS Immunol Med Microbiol 50:1~26
Garate M / 2006 / In vitro pathogenicity of Acanthamoeba is associated with the expression of the mannose-binding protein / Invest Ophthalmol Vis Sci 47:1056~1062
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UCI(KEPA) : I410-ECN-0101-2014-510-002534885