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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 281 - 284 (4page)
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Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples.
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참고문헌 (17)
참고문헌 신청
1. [학술지(정기간행물)] - Haque R / 2006 / Diagnosis of amebiasis in Bangladesh / Arch Med Res 37 : 273 ~ 276
2. [학술지(정기간행물)] - World Health Organization / 1997 / Amoebiasis / WHO Weekly Epidemiol Rec 72 : 97 ~ 100
3. [학술지(정기간행물)] - Gonzalez-Ruiz A / 1994 / Value of microscopy in the diagnosis of dysentery associated with invasive Entamoeba histolytica / J Clin Pathol 47 : 236 ~ 239
4. [학술지(정기간행물)] - Tannich E / 1991 / Differentiation of pathogenic from nonpathogenic Entamoeba histolytica by restriction fragment analysis of a single gene amplified in vitro / J Clin Microbiol 29 : 250 ~ 255
5. [학술지(정기간행물)] - Britten D / 1997 / An improved colorimetric PCR-based method for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in feces / J Clin Microbiol 35 : 1108 ~ 1111
2. [학술지(정기간행물)] - World Health Organization / 1997 / Amoebiasis / WHO Weekly Epidemiol Rec 72 : 97 ~ 100
3. [학술지(정기간행물)] - Gonzalez-Ruiz A / 1994 / Value of microscopy in the diagnosis of dysentery associated with invasive Entamoeba histolytica / J Clin Pathol 47 : 236 ~ 239
4. [학술지(정기간행물)] - Tannich E / 1991 / Differentiation of pathogenic from nonpathogenic Entamoeba histolytica by restriction fragment analysis of a single gene amplified in vitro / J Clin Microbiol 29 : 250 ~ 255
5. [학술지(정기간행물)] - Britten D / 1997 / An improved colorimetric PCR-based method for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in feces / J Clin Microbiol 35 : 1108 ~ 1111
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UCI(KEPA) : I410-ECN-0101-2014-510-002511419