지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.4
- 수록면
- 137 - 145 (9page)
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초록· 키워드
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In order to develop new skin whitening agents, we prepared the CH₂Cl₂ layer (NGC) and BuOH layer (NGB) of 75% EtOH extract of the Nelumbinis nucifera Gaertner. We measured their tyrosinase inhibitory activity in vitro and melanin synthesis inhibitory activity in B16-F1 melanoma cells. They did not show inhibitory activity against mushroom tyrosinase but showed melanin synthesis inhibitory activity in a dose-dependent manner. In a melanin synthesis inhibition assay, NGC and NGB suppressed melanin production up to 52% and 46% at a concentration of 100 μg/mL, respectively. To elucidate the mechanism of the inhibitory effects of NGC and NGB on melanogenesis, we measured the expression of melanogenesis-related proteins by western blot assay. As a result, NGC suppressed the expression of tyrosinase, tyrosinase related protein 1 (TRP-1), tyrosinase related protein 2 (TRP-2), phosphorylated cAMP responsive element binding (p-CREB) protein, and microphthalmia associated transcription factor (MITF). And NGB inhibited the protein expression of tyrosinase and MITF, but had no significant effect on TRP-1, TRP-2, and p-CREB expression. Moreover, NGB increased the expression of phosphorylated extracellular signal-regulated kinase (p-ERK). In addition, we examined the inhibitory effect on the glycosylation of tyrosinase. As a result, NGC and NGB inhibited the activity of α-glucosidase in vitro and the glycosylation of tyrosinase in B16-F1 melanoma cells. From these results, we concluded that NGC and NGB could be used as active ingredients for skin whitening.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
REFERENCES
참고문헌 (60)
참고문헌 신청
1. [학술지(정기간행물)] - Hill, H. Z. / 1998 / Melanin: A two edged sword? / Pigment Cell Res 10 : 158 ~ 161
2. [학술지(정기간행물)] - Seiberg, M. / 2003 / IL-41 the PAR-2 pathway is differentially expressed in skin of color / Pigment Cell Res 16 : 591 ~ 591
3. [학술지(정기간행물)] - Busca, R. / 2000 / Cyclic AMP a key messenger in the regulation of skin pigmentation / Pigment Cell Res 13 : 60 ~ 69
4. [학술지(정기간행물)] - Sassone, C. P. / 1998 / Coupling gene expression to cAMP signalling: Role of CREB and CREM / Int. J. Biochem. Cell B 30 : 27 ~ 38
5. [학술지(정기간행물)] - Bertolotto, C. / 1998 / Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP: pivotal role of M boxes (GTCATGTGCT) and of microphthalmia / Mol. Cell. Biol 18 : 694 ~ 702
2. [학술지(정기간행물)] - Seiberg, M. / 2003 / IL-41 the PAR-2 pathway is differentially expressed in skin of color / Pigment Cell Res 16 : 591 ~ 591
3. [학술지(정기간행물)] - Busca, R. / 2000 / Cyclic AMP a key messenger in the regulation of skin pigmentation / Pigment Cell Res 13 : 60 ~ 69
4. [학술지(정기간행물)] - Sassone, C. P. / 1998 / Coupling gene expression to cAMP signalling: Role of CREB and CREM / Int. J. Biochem. Cell B 30 : 27 ~ 38
5. [학술지(정기간행물)] - Bertolotto, C. / 1998 / Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP: pivotal role of M boxes (GTCATGTGCT) and of microphthalmia / Mol. Cell. Biol 18 : 694 ~ 702
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UCI(KEPA) : I410-ECN-0101-2014-400-002408272