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한국생물공학회 KSBB Journal KSBB Journal 제12권 제2호
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167 - 175 (9page)

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초록· 키워드

The pH of medium changes along with cell growth, caused by the consumption of nutrients and by the production of metabolites. The pH is determined by the equilibrium of the ionic components of the medium. The pH of the medium was calculated from the equations of equilibrium and of the material balance of the ionic components and electro-neutrality theory. Consumption of 3 mM of ammonia was calculated to decrease pH of 0.5 at 10 mM of a phosphate buffered minimal medium. Production of 0.5 g/l of acetic acid was calculated to decrease pH of 2.3 at the same medium. The buffer capacity was significantly increased by the production of organic acid at the pH below 6.5 and was proportional to the concentration of ammonium ion at the pH above 7. Ammonium consumption and Acetic acid production are found out to be the major contributors for the alteration of the pH as well as the buffer capacity of the medium.
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UCI(KEPA) : I410-ECN-0101-2014-400-002859346