메뉴 건너뛰기

ENG

추천
검색
질문

논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국생물공학회 KSBB Journal KSBB Journal 제11권 제3호
발행연도
수록면
270 - 275 (6page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
이 논문의 연구방법이 궁금하신가요?
🏆
연구결과
이 논문의 연구결과가 궁금하신가요?
AI에게 요청하기
추천
검색
질문

초록· 키워드

Heterologous expression of glucose oxidase gene using recombinant yeast has been carried out. Polymerase chain reaction was conducted to obtain the gene encoding glucose oxidase from Aspergillus niger and sequence comparison indicated the cloned 1.9kb DNA fragment appeared to be the glucose oxidase structural gene containing a signal sequence for extracellular location. Transforming shuttle vector was constructed with YEp352 to express the cloned glucose oxidase gene under the control of either GAL1 or GAL10 promoter. Plate assay of recombinant yeasts has shown that GAL1 promoter was more effective in yielding glucose oxidase than GAL10 promoter. Among the five different concentrations of galactose tried, 1% galactose showed the highest induction of glucose oxidase. Cellular localization experiment of recombinant enzyme using spheroplast revealed that most of enzymes(80%) were secreted into culture media in contrast to A. niger. There is no difference in heat-stability of recombinant enzyme up to 50°C compared to the glucose oxidase from A. niger. However, a dramatic reduction of enzyme activity was observed in both enzymes at 60°C.
상세정보 수정요청해당 페이지 내 제목·저자·목차·페이지
정보가 잘못된 경우 알려주세요!

목차

  1. ABSTRACT
  2. 서론
  3. 재료 및 방법
  4. 결과 및 고찰
  5. 요약
  6. 참고문헌

참고문헌

참고문헌 신청

최근 본 자료

전체보기
260319_딥리서치
260319_AI리더
260304_새학기에이전트
260420 이주의질문
260122_AIagent
260304_아그논문롤
260127_아그논문
260127_AI뷰어
UCI(KEPA) : I410-ECN-0101-2014-400-002850212