Overexpression of Ubiquitin and Amino Acid Permease Genes in Association with Antimony Resistance in Leishmania tropica Field Isolates

Elham Kazemi-Rad; Mehdi Mohebali; Mohammad Bagher Khadem-Erfan; Homa Hajjaran; Ramtin Hadighi; Ali Khamesipour; Sassan Rezaie; Mojtaba Saffari; Reza Raoofian; Mansour Heidari

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자료유형: 학술저널

저자정보: Elham Kazemi-Rad (Tehran University of Medical Sciences) Mehdi Mohebali (Tehran University of Medical Sciences) Mohammad Bagher Khadem-Erfan (Tehran University of Medical Sciences) Homa Hajjaran (Tehran University of Medical Sciences) Ramtin Hadighi (Iran University of Medical Sciences) Ali Khamesipour (Tehran University of Medical Sciences) Sassan Rezaie (Tehran University of Medical Sciences) Mojtaba Saffari (Tehran University of Medical Sciences) Reza Raoofian (Tehran University of Medical Sciences) Mansour Heidari (Tehran University of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.51 No.4

발행연도: 2013.8

수록면: 413 - 419 (7page)

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The mainstay therapy against leishmaniasis is still pentavalent antimonial drugs; however, the rate of antimony resistance is increasing in endemic regions such as Iran. Understanding the molecular basis of resistance to antimonials could be helpful to improve treatment strategies. This study aimed to recognize genes involved in antimony resistance of Leishmania tropica field isolates. Sensitive and resistant L. tropica parasites were isolated from anthroponotic cutaneous leishmaniasis patients and drug susceptibility of parasites to meglumine antimoniate (Glucantime<SUP>®</SUP>) was confirmed using in vitro assay. Then, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) and real-time reverse transcriptase-PCR (RT-PCR) approaches were utilized on mRNAs from resistant and sensitive L. tropica isolates. We identified 2 known genes, ubiquitin implicated in protein degradation and amino acid permease (AAP3) involved in arginine uptake. Also, we identified 1 gene encoding hypothetical protein. Real-time RT-PCR revealed a significant upregulation of ubiquitin (2.54-fold), and AAP3 (2.86-fold) (P<0.05) in a resistant isolate compared to a sensitive one. Our results suggest that overexpression of ubiquitin and AAP3 could potentially implicated in natural antimony resistance.

#Leishmania tropica #antimony resistance #cDNA-AFLP #real-time RT-PCR #ubiquitin #amino acid permease

참고문헌 (34)

참고문헌 신청

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