지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.6
- 수록면
- 279 - 287 (9page)
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초록· 키워드
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Autophagy is a process of cytoplasmic degradation of endogenous proteins and organelles. Although its primary role is protective, it can also contribute to cell death. Recently, autophagy was found to play a role in the activation of host defense against intracellular pathogens. The aims of our study was to investigate whether host cell autophagy influences Toxoplasma gondii proliferation and whether autophagy inhibitors modulate cell survival. HeLa cells were infected with T. gondii with and without rapamycin treatment to induce autophagy. Lactate dehydrogenase assays showed that cell death was extensive at 36-48 hr after infection in cells treated with T. gondii with or without rapamycin. The autophagic markers, LC3 II and Beclin 1, were strongly expressed at 18-24 hr after exposure as shown by Western blotting and RT-PCR. However, the subsequent T. gondii proliferation suppressed autophagy at 36 hr post-infection. Pre-treatment with the autophagy inhibitor, 3-methyladenine (3-MA), down-regulated LC3 II and Beclin 1. The latter was also down-regulated by calpeptin, a calpain inhibitor. Monodansyl cadaverine (MDC) staining detected numerous autophagic vacuoles (AVs) at 18 hr post-infection. Ultrastructural observations showed T. gondii proliferation in parasitophorous vacuoles (PVs) coinciding with a decline in the numbers of AVs by 18 hr. FACS analysis failed to confirm the presence of cell apoptosis after exposure to T. gondii and rapamycin. We concluded that T. gondii proliferation may inhibit host cell autophagy and has an impact on cell survival.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (27)
참고문헌 신청
1. [학술지(정기간행물)] - Levine B / 2008 / Bcl-2 family members. Dual regulators of apoptosis and autophagy / Autophagy 4 : 600 ~ 606
2. [학술지(정기간행물)] - Wang J / 2008 / Beclin 1 bridges autophagy, apoptosis and differentiation / Autophagy 4 : 947 ~ 948
3. [학술지(정기간행물)] - Klionsky DJ / 2000 / Autophagy as a regulated pathway of cellular degradation / Science 290 : 1717 ~ 1721
4. [학술지(정기간행물)] - Shen ZY / 2008 / Autophagy and endocytosis in the amnion / J Struct Biol 162 : 197 ~ 204
5. [학술지(정기간행물)] - Bruno P / 2007 / Cell survival under nutrient stress is dependent on metabolic conditions regulated by Akt and not by autophagic vacuoles / Cell Signal 19 : 2118 ~ 2126
2. [학술지(정기간행물)] - Wang J / 2008 / Beclin 1 bridges autophagy, apoptosis and differentiation / Autophagy 4 : 947 ~ 948
3. [학술지(정기간행물)] - Klionsky DJ / 2000 / Autophagy as a regulated pathway of cellular degradation / Science 290 : 1717 ~ 1721
4. [학술지(정기간행물)] - Shen ZY / 2008 / Autophagy and endocytosis in the amnion / J Struct Biol 162 : 197 ~ 204
5. [학술지(정기간행물)] - Bruno P / 2007 / Cell survival under nutrient stress is dependent on metabolic conditions regulated by Akt and not by autophagic vacuoles / Cell Signal 19 : 2118 ~ 2126
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UCI(KEPA) : I410-ECN-0101-2014-510-002777700