지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.10
- 수록면
- 497 - 502 (6page)
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초록· 키워드
오류제보하기
Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cyst-forming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess a type of Atg8. In this study, an isoform of Atg8 was identified and characterized in Acanthamoeba castellanii (AcAtg8b). AcAtg8b protein was found to encode 132 amino acids and to be longer than AcAtg8 protein, which encoded 117 amino acids. Real-time PCR analysis showed high expression levels of AcAtg8b and AcAtg8 during encystation. Fluorescence microscopy demonstrated that AcAtg8b is involved in the formation of the autophagosomal membrane. Chemically synthesized siRNA against AcAtg8b reduced the encystation efficiency of Acanthamoeba, confirming that AcAtg8b, like AcAtg8, is an essential component of cyst formation in Acanthamoeba. Our findings suggest that Acanthamoeba has doubled the number of Atg8 gene copies to ensure the successful encystation for survival when 1 copy is lost. These 2 types of Atg8 identified in Acanthamoeba provide important information regarding autophagy formation, encystation mechanism, and survival of primitive, cyst-forming protozoan parasites.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (20)
참고문헌 신청
Levine B / 2004 / Development by self-digestion : molecular mechanisms and biological functions of autophagy / Dev Cell 6:463~477
Picazarri K / 2008 / Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadens / Infect Immun 76:278~288
Shpilka T / 2011 / Atg8 : an autophagy-related ubiquitin-like protein family / Genome Biol 12:226~236
Suzuki K / 2007 / Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae / FEBS Lett 581:2156~2161
Kuma A / 2002 / Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast / J Biol Chem 277:18619~18625
Picazarri K / 2008 / Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadens / Infect Immun 76:278~288
Shpilka T / 2011 / Atg8 : an autophagy-related ubiquitin-like protein family / Genome Biol 12:226~236
Suzuki K / 2007 / Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae / FEBS Lett 581:2156~2161
Kuma A / 2002 / Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast / J Biol Chem 277:18619~18625
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UCI(KEPA) : I410-ECN-0101-2014-510-003484208