지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.12
- 수록면
- 221 - 227 (7page)
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초록· 키워드
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Embryonic stem (ES) cells have potential for use in evaluation of developmental toxicity because they are generated in large numbers and differentiate into three germ layers following formation of embryoid bodies (EBs). In earlier study, embryonic stem cell test (EST) was established for assessment of the embryotoxic potential of compounds. Using EBs indicating the onset of differentiation of mouse ES cells, many toxicologists have refined the developmental toxicity of a variety of compounds. However, due to some limitation of the EST method resulting from species-specific differences between humans and mouse, it is an incomplete approach. In this regard, we examined the effects of several developmental toxic chemicals on formation of EBs using human ES cells. Although human ES cells are fastidious in culture and differentiation, we concluded that the relevancy of our experimental method is more accurate than that of EST using mouse ES cells. These types of studies could extend our understanding of how human ES cells could be used for monitoring developmental toxicity and its relevance in relation to its differentiation progress. In addition, this concept will be used as a model system for screening for developmental toxicity of various chemicals. This article might update new information about the usage of embryonic stem cells in the context of their possible ability in the toxicological fields.
목차
INTRODUCTION
EMBRYONIC STEM CELL TEST (EST)
DEVELOPMENTAL TOXICANTS ON DIFFERENTIATED MOUSE ES CELLS
DEVELOPMENTAL TOXICANTS ON UNDIFFERENTIATED HUMAN ES CELLS
DEVELOPMENTAL TOXICANTS ON DIFFERENTIATED ES CELLS
PERSPECTIVES
REFERENCES
참고문헌 (65)
참고문헌 신청
Höfer, T. I. / 2004 / Animal testing and alternative approaches for the human health risk assessment under the proposed new European chemicals regulation / Arch. Toxicol. 78:549~564
Yamanaka, S. / 2008 / Pluripotency of embryonic stem cells / Cell Tissue Res. 331:5~22
Heuer, J. / 1993 / Development of an in vitro embryotoxicity test using murine embryonic stem cell cultures / Toxicol. In Vitro 7:551~556
Scholz, G. / 1999 / Embryotoxicity screening using embryonic stem cells in vitro: correlation to in vivo teratogenicity / Cells Tissues Organs 165:203~211
Genschow, E. / 1999 / Development of prediction models for three in vitro embryotoxicity tests which are evaluated in an ECVAM validation study / ALTEX 16:73~83
Yamanaka, S. / 2008 / Pluripotency of embryonic stem cells / Cell Tissue Res. 331:5~22
Heuer, J. / 1993 / Development of an in vitro embryotoxicity test using murine embryonic stem cell cultures / Toxicol. In Vitro 7:551~556
Scholz, G. / 1999 / Embryotoxicity screening using embryonic stem cells in vitro: correlation to in vivo teratogenicity / Cells Tissues Organs 165:203~211
Genschow, E. / 1999 / Development of prediction models for three in vitro embryotoxicity tests which are evaluated in an ECVAM validation study / ALTEX 16:73~83
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UCI(KEPA) : I410-ECN-0101-2015-510-001071714