Purpose: This study was conducted to investigate the correlation between uncertainty and depression in hospitalized children"s mothers. Methods: The subjects were 175 mothers of children who were hospitalized in pediatric unit. A self report questionnaires were used to measure the variables. Variables were uncertainty and depression. In data analysis, SPSSWIN 20.0 program was utilized for descriptive statistics, ANOVA, Pearson"s correlation coefficients and multiple regression. Results: Reliability of instruments were found cronbach"s alpha=.85~.86. Uncertainty correlated positively with depression(r=.422, p<.010). There were significant differences in depression according to birth order(F=6.58, p=.002), mothers" age(F=6.57, p=.002), education level(F=3.97, p=.021) and mothers" job(t=19.18, p<.001), monthly income(F=4.234, p=.006) and family type(t=7.49, p=.007). In multiple regression, uncertainty, mothers" job and family type were significant predictors of depression in hospitalized children"s mothers explaining 30.9%. Conclusions: Uncertainty and depression were significant variables in hospitalized children"s mothers. The strategy of nursing intervention which decrease depression in mothers must be developed by decreasing level of uncertainty in mothers of hospitalized children.