Circulating tumor cells (CTCs) present in the bloodstream of cancer patients. CTCs provide a potentially accessible source for detection, characterization, and monitoring of nonhematological cancers. We demonstrate the effectiveness of the CTC-Chip for the isolation of ovarian cancer cells by adapting the herringbone-chip (HB-Chip). The motion of the particles on the HB chip are simulated by a unique combination of buoyant, gravitational forces, and helical flows with computational modeling. The real motion of cells are demonstrated by applying polystylene bead and ovarian cancer cells into the microfabricated HB chip. The experimental results from beads and cells are well accordance with the simulated ones, as previous reported by Toner group. Thus, we expect that these modeling and experimental skills will play key roles in the clinical applications on CTC isolation as well as the basic research on characterization of CTCs under flow.