지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Hypoxia is one of the main reasons for islet apoptosis after transplantation as well as during isolation. In this study, we attempted to determine the potential usefulness of NF-κB inhibitor for suppression of hypoxia-induced β-cell apoptosis as well as the relationship between IP-10 induction and β-cell apoptosis in hypoxia. To accomplish this, we cultured the mouse pancreatic β-cell line MIN6 in hypoxia (1% O₂). Among several examined chemokines, only IP-10 mRNA expression was induced under hypoxia, and this induced IP-10 expression was due to NF-κB activity. Since a previous study suggested that IP-10 mediates β-cell apoptosis, we measured hypoxia-induced IP-10 protein and examined the effect of anti-IP-10 neutralizing Ab on hypoxia-induced β-cell apoptosis. However, IP-10 protein was not detected, and anti-IP-10 neutralizing Ab did not rescue hypoxia-induced MIN6 apoptosis, indicating that there is no relationship between hypoxia-induced IP-10 mRNA expression and hypoxia-induced β-cell apoptosis. Since it was still not clear if NF-κB functions as an apoptotic or anti-apoptotic mediator in hypoxia-induced β-cell apoptosis, we examined possible involvement of NF-κB in hypoxia-induced β-cell apoptosis. Treatment with 1 μM NF-κB inhibitor suppressed hypoxiainduced apoptosis by more than 50%, while 10 μM AP-1 or 4 μM NF-AT inhibitor did not, indicating involvement of NF-κB in hypoxia-induced β-cell apoptosis. Overall, these results suggest that IP-10 is not involved in hypoxia-induced β-cell apoptosis, and that NF-κB inhibitor can be useful for ameliorating hypoxia-induced β-cell apoptosis.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (52)
참고문헌 신청
1. [학술지(정기간행물)] - Aida K / 2011 / RIG-I- and MDA5-initiated innate immunity linked with adaptive immunity accelerates β-cell death in fulminant type 1 diabetes / Diabetes 60 : 884 ~ 889
2. [학술지(정기간행물)] - Baker MS / 2003 / Proinflammatory cytokines induce NF-κB-dependent/NOindependent chemokine gene expression in MIN6 β cells / J Surg Res 110 : 295 ~ 303
3. [학술지(정기간행물)] - Barshes NR / 2004 / Achievement of insulin independence via pancreatic islet transplantation using a remote isolation center: A first-year review / Transplant Proc 36 : 1127 ~ 1129
4. [학술지(정기간행물)] - Buttmann M / 2007 / Interferon-γ is a potent inducer of interferon regulatory factor-1/2-dependent IP-10/CXCL10 expression in primary human endothelial cells / J Vasc Res 44 : 51 ~ 60
5. [학술지(정기간행물)] - Cantley J / 2010 / The hypoxia response pathway and β-cell function / Diabetes Obes Metabol 12 : 159 ~ 167
2. [학술지(정기간행물)] - Baker MS / 2003 / Proinflammatory cytokines induce NF-κB-dependent/NOindependent chemokine gene expression in MIN6 β cells / J Surg Res 110 : 295 ~ 303
3. [학술지(정기간행물)] - Barshes NR / 2004 / Achievement of insulin independence via pancreatic islet transplantation using a remote isolation center: A first-year review / Transplant Proc 36 : 1127 ~ 1129
4. [학술지(정기간행물)] - Buttmann M / 2007 / Interferon-γ is a potent inducer of interferon regulatory factor-1/2-dependent IP-10/CXCL10 expression in primary human endothelial cells / J Vasc Res 44 : 51 ~ 60
5. [학술지(정기간행물)] - Cantley J / 2010 / The hypoxia response pathway and β-cell function / Diabetes Obes Metabol 12 : 159 ~ 167
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UCI(KEPA) : I410-ECN-0101-2015-500-001321419