Hydro-acoustic survey was used to analyze spatio-temporal distribution of fish school in the artificial reef area. The survey was carried out in Uljin on east coast of Korea in 22-23 September 2012. A track survey was setting 9 research lines where artificial reef area. The survey system was set up with GPS system (GP-50, Furuno), transducer (DT-5000, BioSonics), quantitative echo sounder and echoview (3.00 Myriax). As a result, spatio-temporal distribution of fish school where artificial reef area are spread on coast at afternoon-sunset, dawn-sunrise and morning and in the whole sea at night. The density of fish schools are highly distributed on the basis of time in order of dawn-sunrise, night, morning and afternoon-sunset. The fish school are distributed 20-25 m at afternoon-sunset, 5-20 m at night, 0-15 m at dawn-sunrise and 0-10 m at morning. Moreover, this survey result can be used as an information to evaluate marine resources inhabiting in the sea around artificial reef.