지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.12
- 수록면
- 9 - 16 (8page)
이용수
초록· 키워드
오류제보하기
Objectives We investigated the genotoxic effects of 40-59 ㎚ silver nanoparticles (Ag-NPs) by bacterial reverse mutation assay (Ames test), in vitro comet assay and micronucleus (MN) assay. In particular, we directly compared the effect of cytochalasin B (cytoB) and rat liver homogenate (S9 mix) in the formation of MN by Ag-NPs.
Methods Before testing, we confirmed that Ag-NPs were completely dispersed in the experimental medium by sonication (three times in 1 minute) and filtration (0.2 ㎛ pore size filter), and then we measured their size in a zeta potential analyzer. After that the genotoxicity were measured and especially, S9 mix and with and without cytoB were compared one another in MN assay.
Results Ames test using Salmonella typhimurium TA98, TA100, TA1535 and TA1537 strains revealed that Ag-NPs with or without S9 mix did not display a mutagenic effect. The genotoxicity of Ag-NPs was also evaluated in a mammalian cell system using Chinese hamster ovary cells. The results revealed that Ag-NPs stimulated DNA breakage and MN formation with or without S9 mix in a dose-dependent manner (from 0.01 ㎍/mL to 10 ㎍/mL). In particular, MN induction was affected by cytoB.
Conclusions All of our findings, with the exception of the Ames test results, indicate that Ag-NPs show genotoxic effects in mammalian cell system. In addition, present study suggests the potential error due to use of cytoB in genotoxic test of nanoparticles.
Methods Before testing, we confirmed that Ag-NPs were completely dispersed in the experimental medium by sonication (three times in 1 minute) and filtration (0.2 ㎛ pore size filter), and then we measured their size in a zeta potential analyzer. After that the genotoxicity were measured and especially, S9 mix and with and without cytoB were compared one another in MN assay.
Results Ames test using Salmonella typhimurium TA98, TA100, TA1535 and TA1537 strains revealed that Ag-NPs with or without S9 mix did not display a mutagenic effect. The genotoxicity of Ag-NPs was also evaluated in a mammalian cell system using Chinese hamster ovary cells. The results revealed that Ag-NPs stimulated DNA breakage and MN formation with or without S9 mix in a dose-dependent manner (from 0.01 ㎍/mL to 10 ㎍/mL). In particular, MN induction was affected by cytoB.
Conclusions All of our findings, with the exception of the Ames test results, indicate that Ag-NPs show genotoxic effects in mammalian cell system. In addition, present study suggests the potential error due to use of cytoB in genotoxic test of nanoparticles.
목차
Introduction
Materials and Methods
Results
Discussion
References
참고문헌 (38)
참고문헌 신청
Kirkland D / Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens I. Sensitivity, specificity and relative predictivity / Mutat Res 584(1-2):1~256
Warheit DB / 2010 / Rationale of genotoxicity testing of nanomaterials: regulatory requirements and appropriateness of available OECD test guidelines / Nanotoxicology 4:409~413
Ames BN / 1973 / An improved bacterial test system for the detection and classification of mutagens and carcinogens / Proc Natl Acad Sci USA 70(3):782~786
Landsiedel R / 2009 / Genotoxicity investigations on nanomaterials: methods, preparation and characterization of test material, potential artifacts and limitations-- many questions, some answers / Mutat Res 681(2-3):241~258
Kisin ER / 2007 / Single-walled carbon nanotubes: geno- and cytotoxic effects in lung fibroblast V79 cells / J Toxicol Environ Health A 70(24):2071~2079
Warheit DB / 2010 / Rationale of genotoxicity testing of nanomaterials: regulatory requirements and appropriateness of available OECD test guidelines / Nanotoxicology 4:409~413
Ames BN / 1973 / An improved bacterial test system for the detection and classification of mutagens and carcinogens / Proc Natl Acad Sci USA 70(3):782~786
Landsiedel R / 2009 / Genotoxicity investigations on nanomaterials: methods, preparation and characterization of test material, potential artifacts and limitations-- many questions, some answers / Mutat Res 681(2-3):241~258
Kisin ER / 2007 / Single-walled carbon nanotubes: geno- and cytotoxic effects in lung fibroblast V79 cells / J Toxicol Environ Health A 70(24):2071~2079
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2015-530-001447549