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논문 기본 정보

자료유형
학술저널
저자정보
Ji Soo Kim (Chonnam National University) Yong Hwan Kim (Korea Institute of Planning & Evaluation for Technology on Food, Agriculture, Forestry & Fishe) Anne J. Anderson (Utah State University) Young Cheol Kim (Chonnam National University)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.30 No.2
발행연도
2014.5
수록면
215 - 219 (5page)

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The GacS/GacA two component system regulates various traits related to the biocontrol potential of plant-associated pseudomonads. The role of the sensor kinase, GacS, differs between strains in regulation of motility. In this study, we determined how a gacS mutation changed cell morphology and motility in Pseudomonas chlororaphis O6. The gacS mutant cells were elongated in stationaryphase compared to the wild type and the complemented gacS mutant, but cells did not differ in length in logarithmic phase. The gacS mutant had a two-fold increase in the number of flagella compared with the wild type strain; flagella number was restored to that of the wild type in the complemented gacS mutant. The more highly flagellated gacS mutant cells had greater swimming motilities than that of the wild type strain. Enhanced flagella formation in the gacS mutant correlated with increased expression of three genes, fleQ, fliQ and flhF, involved in flagellar formation. Expression of these genes in the complemented gacS mutant was similar to that of the wild type. These findings show that this root-colonizing pseudomonad adjusts flagella formation and cell morphology in stationary-phase using GacS as a major regulator.

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