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논문 기본 정보

자료유형
학술저널
저자정보
Guochao Zheng (South China Agricultural University) Muhamd Alsarakibi (South China Agricultural University) Yuanjia Liu (South China Agricultural University) Wei Hu (South China Agricultural University) Qin Luo (South China Agricultural University) Liping Tan (South China Agricultural University) Guoqing Li (South China Agricultural University)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.52 No.3
발행연도
2014.6
수록면
299 - 304 (6page)

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This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs’ fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.

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