Purpose: The purpose of this study was to identify factors influencing workplace bullying among Korean hospital nurses. Methods: Subjects in this study included 178 hospital nurses who attended two nationwide nursing educational conferences in 2013. The data was collected using self-report questionnaires which were used to identify the subjects’ characteristics, self-esteem, perception of nursing organizational culture, and workplace bullying experience. Results: The results showed that 19.1% of study subjects reported being victims of workplace bullying and mostly experienced person-related and work-related bullying. Multiple regression analysis was done to identify factors affecting workplace bullying. The perception of relation-oriented culture, task-oriented culture and self-esteem turned out to be variables that explained nurses’ workplace bullying, and these factors accounted for 32.0% of the variance. Conclusion: The findings suggest that nurses experience more workplace bullying in task-oriented cultures. However, they experience less bullying and report higher self-esteem and awareness in relation-oriented cultures. Interventions focused on the characteristics of the organization need to be developed to prevent workplace bullying in hospital nurses.