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논문 기본 정보

자료유형
학술저널
저자정보
Mi-Sun Yoo (Animal and Plant Quarantine Agency) Ha-Na Jung (Animal and Plant Quarantine Agency) Thi Bich Thao Le (Vietnam Academy of Science and Technology) Young-Ha Kim (Animal and Plant Quarantine Agency) Nam-Hee Kim (Animal and Plant Quarantine Agency) Kondreddy Eswar Reddy (Animal and Plant Quarantine Agency) Jin-Hyeong Noh (Animal and Plant Quarantine Agency) Huong Thi Thanh Doan (Vietnam Academy of Science and Technology) Lien Thi Kim Nguyen (Vietnam Academy of Science and Technology) Suk-Chan Jung (Animal and Plant Quarantine Agency) Seung-Won Kang (Animal and Plant Quarantine Agency)
저널정보
한국양봉학회 Journal of Apiculture Journal of Apiculture Vol.30 No.1
발행연도
2015.4
수록면
21 - 30 (10page)
DOI
10.17519/apiculture.2015.04.30.1.21

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초록· 키워드

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Recently, the Korean sacbrood virus (KSBV) caused great loss of honeybee colonies in Korean Apis mellifera. Three antimicrobial peptide genes (AMPs) (abaecin (ABA), defensin (DEF) and hymenoptaecin (HYM)) and two immune-related enzyme genes (phenol-oxidase (PO) and lysozyme (LYS)) were investigated using qRT-PCR as a measure of the defense reactions of honeybee larvae and adults challenged with KSBV in vitro. Transcript levels of three AMPs were upregulated in adults exposed to KSBV, compared to control adults. ABA and DEF transcripts were 22.7 and 21.9 fold higher (P<0.05), respectively, after 24h exposure while HYM transcript did not change significantly. At this time point, PO and LYS mRNA levels were 18.08 and 16.03 fold higher, respectively, in KSBVinfected adult bees compared to control adults (P<0.05). By contrast, the larvae infected with KSBV had downregulated expression of AMPs and slight up-regulation of PO and LYS compared to the controls. These results suggest that the differential expression of the genes studied could indicate their involvement in defense processes against KSBV in adult and larva bees.

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Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
LITERATURE CITED

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