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학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제21권 제1호
발행연도
1991.6
수록면
49 - 55 (7page)

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HFRS vaccine inactivated Hantaan virus with formalin prepared from infected suckling rat brain was developed in Korea, the antigenic potency of the vaccine and the Hantaan virus infectivity challenge test was studied using hamster as a model to assay induction of protective immunity by HFRS vaccine. 8-10 weeks old seronegative against Hantaan virus normal hamsters were immunized with 4096 ELISA unit of vaccine by intraperitoneal route two times at a 10days intervals, then were challenged 7 days after booster injection with live Hantaan virus (5 x 10 PFU). The animals were bled by cardiac puncture 3 weeks after virus challenge and various organs were collected for antigen detection using indirect immunofluorescence test (IFT) and Enzyme linked immunosorbent assay (ELISA). Mean antibody titer against Hantaan virus after vaccination was 1:210 by High density particle agglutination (HDPA) test and the neutralizing antibody titer was 1:16 by plaque reduction neutralization test (PRNT). Hantaan virus antigen was detected in the lung of hamster 3 weeks after virus challenge, however the antigen was not detected in the lung of vaccinated hamster. Above result suggested that HFRS inactivated vaccine was effective to production of neutralizing antibody and to protection against Hantaan virus in experimental animal.

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