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논문 기본 정보

자료유형
학술저널
저자정보
Ja Yeol Lee (Seokyeong University) Doo Hyun Park (Seokyeong University)
저널정보
대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY Journal of Bacteriology and Virology 제42권 제4호
발행연도
2012.12
수록면
313 - 320 (8page)

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초록· 키워드

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The purpose of this study was to examine profile of bacteria contaminated in a dry sauna. Bacteria sampled from the floor of the dry sauna (75~80℃) were separated and identified by using a thermal gradient gel electrophoresis (TGGE) technique, and were cultivated using a defined medium at 50℃. Bacteria grown in the defined medium were identified based on 16S-rDNA sequence homology. The band number of DNA separated by TGGE was 15, indicating the species diversity of bacteria contaminating the dry sauna. Seven species of bacteria were successfully cultured on agar plate medium at 50℃, which represented a combination of thermophilic and thermoduric bacteria contaminating the dry sauna. The highest limit temperature for growth of the bacterial isolates was generally 50℃ when cultivated in a defined medium, but was raised to 60℃ when cultivated in a complex medium. Consequently, the bacteria grown at 50~60℃ are thermoduric or thermophilic, but others may not be.

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INTRODUCTION
MATERIALS AND MOTHODS
RESULTS
DISCUSSION
REFERENCES

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