지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.4
- 수록면
- 114 - 122 (9page)
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The leukocyte common antigen (CD45) is a transmembrane-type protein tyrosine phosphatase that has five isoforms. Methods: We generated seven murine mAbs against human CD45 by injecting cells from different origins, such as human thymocytes, PBMCs, and leukemic cell lines. By using various immunological methods including flow cytometry, immunohistochemistry, and immunoprecipitation, we evaluated the reactivity of those mAbs to CD45 of thymus as well as tonsil lysates. Furthermore, we transiently transfected COS-7 cells with each of gene constructs that express five human CD45 isoforms respectively, and examined the specificities of the mAbs against the transfected isoforms. Results: In case of thymocytes, lymphocytes, and monocytes, all the seven mAbs demonstrated positive reactivities whereas none was reactive to erythrocytes and platelets. The majority of immune cells in formalin-fixed paraffin-embedded thymus and tonsil tissues displayed strong membranous immunoreactivity, and the main antigen was detected near 220 kDa in all cases. Among the mAbs, four mAbs (AP4, DN11, SHL-1, and P6) recognized a region commonly present in all the five isoforms. One mAb, YG27, recognized four isoforms (ABC, AB, BC, and O). Two mAbs, P1 and P14, recognized the isoforms that contain exon A encoded regions (ABC and AB). Conclusion: In this study, we confirmed that AP4, DN11, SHL-1, YG27 and P6, are mAbs reactive with the CD45 antigen whereas P1 and P14 are reactive with the CD45RA antigen.
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참고문헌 (25)
참고문헌 신청
1. [학술지(정기간행물)] - Thomas ML / 1989 / The leukocyte common antigen family / Annu Rev Immunol 7 : 339 ~ 369
2. [학술지(정기간행물)] - Trowbridge IS / 1994 / CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development / Annu Rev Immunol 12 : 85 ~ 116
3. [학술지(정기간행물)] - Omary MB / 1980 / Human homologue of murine T200 glycoprotein / J Exp Med 152 : 842 ~ 852
4. [학술지(정기간행물)] - Andersson LC / 1980 / Molecular identification of T cell-specific antigens on human T lymphocytes and thymocytes / Eur J Immunol 10 : 359 ~ 362
5. [학술지(정기간행물)] - Dalchau R / 1980 / Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat / Eur J Immunol 10 : 737 ~ 744
2. [학술지(정기간행물)] - Trowbridge IS / 1994 / CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development / Annu Rev Immunol 12 : 85 ~ 116
3. [학술지(정기간행물)] - Omary MB / 1980 / Human homologue of murine T200 glycoprotein / J Exp Med 152 : 842 ~ 852
4. [학술지(정기간행물)] - Andersson LC / 1980 / Molecular identification of T cell-specific antigens on human T lymphocytes and thymocytes / Eur J Immunol 10 : 359 ~ 362
5. [학술지(정기간행물)] - Dalchau R / 1980 / Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat / Eur J Immunol 10 : 737 ~ 744
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