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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.12
- 수록면
- 296 - 300 (5page)
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초록· 키워드
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Silica nanoparticles, which are applicable in many industrial fields, have been reported to induce cellular changes such as cytotoxicity in various cells and fibrosis in lungs. Because the immune system is the primary targeting organ reacting to internalized exogenous nanoparticles, we tried to figure out the immunostimulatory effect of silica nanoparticles in macrophages using differently sized silica nanoparticles. Using U937 cells we assessed cytotoxicity by CCK-8 assay, ROS generation by CM-H<sub>2</sub>DCFDA, intracellular Ca<sup>{+}{+}</sup> levels by staining with Fluo4-AM and IL-8 production by ELISA. At non-toxic concentration, the intracellular Ca<sup>{+}{+}</sup> level has increased immediately after exposure to 15 nm particles, not to larger particles. ROS generation was detected significantly in response to 15 nm particles. However, all three different sizes of silica nanoparticles induced IL-8 production. 15 nm silica nanoparticles are more stimulatory than larger particles in cytotoxicity, intracellular Ca<sup>{+}{+}</sup> increase and ROS generation. But IL-8 production was induced to same levels with 50 or 100 nm particles. Therefore, IL-8 production induced by silica nanoparticles may be dependent on other mechanisms rather than intracellular Ca<sup>{+}{+}</sup> increase and ROS generation.
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참고문헌 (13)
참고문헌 신청
1. [학술지(정기간행물)] - Feng, X. / 2006 / Converting ceria polyhedral nanoparticles into single-crystal nanospheres / Science 312 : 1504 ~ 1508
2. [학술지(정기간행물)] - Anas, A. / / Sequential interactions of silver- silica nanocomposite (Ag-SiO(2) NC) with cell wall, metabolism and genetic stability of Pseudomonas aeruginosa, a multiple antibiotic-resistant bacterium / Lett. Appl. Microbiol 56 : 57 ~ 62
3. [학술지(정기간행물)] - Li, X. / 2013 / A mesoporous silica nanoparticle - PEI - Fusogenic peptide system for siRNA delivery in cancer therapy / Biomaterials 34 : 1391 ~ 1401
4. [학술지(정기간행물)] - Scalia, S. / 2012 / Comparative Evaluation of the Effect of PermeationEnhancers, Lipid Nanoparticles and Colloidal Silica on in vivoHuman Skin Penetration of Quercetin / Skin Pharmacol.Physiol 26 : 57 ~ 67
5. [학술지(정기간행물)] - 김승재 / 2012 / Phagocytosis and Endocytosis of Silver Nanoparticles Induce Interleukin-8 Production in Human Macrophages / Yonsei Medical Journal 53 (3) : 654 ~ 657
2. [학술지(정기간행물)] - Anas, A. / / Sequential interactions of silver- silica nanocomposite (Ag-SiO(2) NC) with cell wall, metabolism and genetic stability of Pseudomonas aeruginosa, a multiple antibiotic-resistant bacterium / Lett. Appl. Microbiol 56 : 57 ~ 62
3. [학술지(정기간행물)] - Li, X. / 2013 / A mesoporous silica nanoparticle - PEI - Fusogenic peptide system for siRNA delivery in cancer therapy / Biomaterials 34 : 1391 ~ 1401
4. [학술지(정기간행물)] - Scalia, S. / 2012 / Comparative Evaluation of the Effect of PermeationEnhancers, Lipid Nanoparticles and Colloidal Silica on in vivoHuman Skin Penetration of Quercetin / Skin Pharmacol.Physiol 26 : 57 ~ 67
5. [학술지(정기간행물)] - 김승재 / 2012 / Phagocytosis and Endocytosis of Silver Nanoparticles Induce Interleukin-8 Production in Human Macrophages / Yonsei Medical Journal 53 (3) : 654 ~ 657
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