지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.6
- 수록면
- 164 - 170 (7page)
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초록· 키워드
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JL1, a specific epitope on CD43, is a potential biomarker for the diagnosis of acute leukemia. Although qualitative assays for detecting leukemia-specific CD43 exist, there is a need to develop quantitative assays for the same. Here, we developed two novel monoclonal antibodies (mAbs), 2C8 and 8E10, recognizing different epitopes on CD43. These clones are capable of pairing with YG5, another mAb against JL1 epitope, because they were selectively obtained using sandwich ELISA. Antigens recognized by 2C8 and 8E10 were confirmed as CD43 by western blotting using the CD43-hFC recombinant protein. When expression on various leukemic cell lines was investigated, 2C8 and 8E10 displayed a disparity in the distribution of the epitope. Enzyme assays revealed that these mAbs recognized a sialic acid-dependent epitope on CD43. Using normal thymus and lymph node paraffin-embedded tissues, we confirmed a difference in the epitopes recognized by the two mAbs that was predicted based on the maturity of the cells in the tissue. In summary, we developed and characterized two mAbs, 2C8 and 8E10, which can be used with YG5 in a sandwich ELISA for detecting leukemia-specific CD43.
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참고문헌 (19)
참고문헌 신청
1. [단행본] - Remold-O'Donnell, E. / 1995 / Leukocyte typing V: white cell differentiation antigens / Oxford University Press : 1697 ~ 1701
2. [학술지(정기간행물)] - Carlsson, S. R. / 1986 / Isolation and characterization of leukosialin, a major sialoglycoprotein on human leukocytes / J. Biol. Chem 251 : 12779 ~ 12786
3. [학술지(정기간행물)] - Shelley, C. S. / 1989 / Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott- Aldrich syndrome. Proc / Natl. Acad. Sci. USA 86 : 2819 ~ 2823
4. [학술지(정기간행물)] - Manjunath, N. / 2002 / Negative regulation of T-cell adhesion and activation by CD43 / Nature 377 : 535 ~ 539
5. [학술지(정기간행물)] - Ardman, B / 1992 / CD43 interferes with T-lymphocyte adhesion / Proc. Natl. Acad. Sci. USA 89 : 5001 ~ 5005
2. [학술지(정기간행물)] - Carlsson, S. R. / 1986 / Isolation and characterization of leukosialin, a major sialoglycoprotein on human leukocytes / J. Biol. Chem 251 : 12779 ~ 12786
3. [학술지(정기간행물)] - Shelley, C. S. / 1989 / Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott- Aldrich syndrome. Proc / Natl. Acad. Sci. USA 86 : 2819 ~ 2823
4. [학술지(정기간행물)] - Manjunath, N. / 2002 / Negative regulation of T-cell adhesion and activation by CD43 / Nature 377 : 535 ~ 539
5. [학술지(정기간행물)] - Ardman, B / 1992 / CD43 interferes with T-lymphocyte adhesion / Proc. Natl. Acad. Sci. USA 89 : 5001 ~ 5005
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