지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 314 - 318 (5page)
이용수
초록· 키워드
오류제보하기
Multilocus sequence typing (MLST) of Salmonella is useful method for replacing serotyping using antisera but is limited by difficulties associated with in polymerase chain reaction (PCR). We optimized the PCR reaction, especially annealing temperature and extension time (94℃ for 2 min; 40 cycles at 94℃ for 30 sec, 56.8℃ for 1 min, 72℃ for 2 min; and 72℃ for 10 min). The degradation of PCR product by thermostable nucleases was inhibited by using template DNAs treated proteinase K or purified by a commercialized preparation kit. The resulting modified MLST was used as accurate and fast typing method.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (10)
참고문헌 신청
[인터넷자원] - Grimont PAD / / Antigenic formulae of the Salmonella serovars. 9th ed. Paris, France: WHO Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur website
[학술지(정기간행물)] - Fitzgerald C / 2007 / Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups / J Clin Microbiol 45 : 3323 ~ 3334
[학술지(정기간행물)] - O'Farrell B / 2012 / Transforming microbial genotyping: A robotic pipeline for genotyping bacterial strains / PLoS One 7 : e48022 ~
[학술지(정기간행물)] - Gibson JR / 1993 / PCR products generated from unpurified Salmonella DNA are degraded by thermostable nuclease activity / Lett Appl Microbiol 16 : 59 ~ 61
[학술지(정기간행물)] - Dahiya S / 2013 / Multiple locus sequence typing of Salmonella Typhi, isolated in north India-a preliminary study / Indian J Med Res 137 : 957 ~ 962
[학술지(정기간행물)] - Fitzgerald C / 2007 / Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups / J Clin Microbiol 45 : 3323 ~ 3334
[학술지(정기간행물)] - O'Farrell B / 2012 / Transforming microbial genotyping: A robotic pipeline for genotyping bacterial strains / PLoS One 7 : e48022 ~
[학술지(정기간행물)] - Gibson JR / 1993 / PCR products generated from unpurified Salmonella DNA are degraded by thermostable nuclease activity / Lett Appl Microbiol 16 : 59 ~ 61
[학술지(정기간행물)] - Dahiya S / 2013 / Multiple locus sequence typing of Salmonella Typhi, isolated in north India-a preliminary study / Indian J Med Res 137 : 957 ~ 962
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UCI(KEPA) : I410-ECN-0101-2016-475-002249972