지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.92 No.2
- 발행연도
- 2017.2
- 수록면
- 67 - 72 (6page)
이용수
초록· 키워드
오류제보하기
Purpose: The major problem in producing artificial livers is that primary hepatocytes cannot be cultured for many days. Recently, 3-dimensional (3D) printing technology draws attention and this technology regarded as a useful tool for current cell biology. By using the 3D bio-printing, these problems can be resolved.
Methods: To generate 3D bio-printed structures (25 mm × 25 mm), cells-alginate constructs were fabricated by 3D bio-printing system. Mouse primary hepatocytes were isolated from the livers of 6–8 weeks old mice by a 2-step collagenase method. Samples of 4 × 10<SUP>7</SUP> hepatocytes with 80%–90% viability were printed with 3% alginate solution, and cultured with well-defined culture medium for primary hepatocytes. To confirm functional ability of hepatocytes cultured on 3D alginate scaffold, we conducted quantitative real-time polymerase chain reaction and immunofluorescence with hepatic marker genes.
Results: Isolated primary hepatocytes were printed with alginate. The 3D printed hepatocytes remained alive for 14 days. Gene expression levels of Albumin , HNF-4α and Foxa3 were gradually increased in the 3D structures. Immunofluorescence analysis showed that the primary hepatocytes produced hepatic-specific proteins over the same period of time.
Conclusion: Our research indicates that 3D bio-printing technique can be used for long-term culture of primary hepatocytes. It can therefore be used for drug screening and as a potential method of producing artificial livers.
Methods: To generate 3D bio-printed structures (25 mm × 25 mm), cells-alginate constructs were fabricated by 3D bio-printing system. Mouse primary hepatocytes were isolated from the livers of 6–8 weeks old mice by a 2-step collagenase method. Samples of 4 × 10<SUP>7</SUP> hepatocytes with 80%–90% viability were printed with 3% alginate solution, and cultured with well-defined culture medium for primary hepatocytes. To confirm functional ability of hepatocytes cultured on 3D alginate scaffold, we conducted quantitative real-time polymerase chain reaction and immunofluorescence with hepatic marker genes.
Results: Isolated primary hepatocytes were printed with alginate. The 3D printed hepatocytes remained alive for 14 days. Gene expression levels of Albumin , HNF-4α and Foxa3 were gradually increased in the 3D structures. Immunofluorescence analysis showed that the primary hepatocytes produced hepatic-specific proteins over the same period of time.
Conclusion: Our research indicates that 3D bio-printing technique can be used for long-term culture of primary hepatocytes. It can therefore be used for drug screening and as a potential method of producing artificial livers.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (15)
참고문헌 신청
[학술지(정기간행물)] - Yu SJ. / 2016 / A concise review of updated guidelines regarding the management of hepatocellular carcinoma around the world : 2010-2016 / Clin Mol Hepatol 22 : 7 ~ 17
[학술지(정기간행물)] - Vinken M / 2014 / Primary hepatocytes and their cultures in liver apoptosis research / Arch Toxicol 88 : 199 ~ 212
[학술지(정기간행물)] - Mandrycky C / 2016 / 3D bioprinting for engineering complex tissues / Biotechnol Adv 34 : 422 ~ 434
[학술지(정기간행물)] - Klaunig JE / 1981 / Mouse liver cell culture. I. Hepatocyte isolation / In Vitro 17 : 913 ~ 925
[학술지(정기간행물)] - Swift B / 2010 / Influence of seeding density and extracellular matrix on bile Acid transport and mrp4 expression in sandwich-cultured mouse hepatocytes / Mol Pharm 7 : 491 ~ 500
[학술지(정기간행물)] - Vinken M / 2014 / Primary hepatocytes and their cultures in liver apoptosis research / Arch Toxicol 88 : 199 ~ 212
[학술지(정기간행물)] - Mandrycky C / 2016 / 3D bioprinting for engineering complex tissues / Biotechnol Adv 34 : 422 ~ 434
[학술지(정기간행물)] - Klaunig JE / 1981 / Mouse liver cell culture. I. Hepatocyte isolation / In Vitro 17 : 913 ~ 925
[학술지(정기간행물)] - Swift B / 2010 / Influence of seeding density and extracellular matrix on bile Acid transport and mrp4 expression in sandwich-cultured mouse hepatocytes / Mol Pharm 7 : 491 ~ 500
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UCI(KEPA) : I410-ECN-0101-2017-514-002372245