지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 87 - 95 (9page)
이용수
초록· 키워드
오류제보하기
It was previously observed that recombinant flock house virus (FHV) RNA1 was efficiently packaged into turnip yellow mosaic virus (TYMV), provided that the TYMV coat protein (CP) sequence was present at the 3"-end. FHV RNA encapsidated by TYMV CPs also had a four-nucleotide extension at the 5"-end. Since even a short extension at the 5"- and 3"-ends of FHV RNA1 inhibits replication, we examined whether the recombinant FHV RNA is indeed capable of replication. To this end, we introduced constructs expressing recombinant FHV RNAs into the plant Nicotiana benthamiana. Northern blot analysis of inoculated leaves suggested abundant production of recombinant FHV RNA1 and its subgenomic RNA. This demonstrated that recombinant FHV RNA with terminal extensions at both ends was competent for replication. We also showed that the recombinant FHV RNA can express the reporter gene encoding enhanced green fluorescent protein.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (20)
참고문헌 신청
Geall AJ, Mandl CW, Ulmer JB. RNA: The new revolution in nucleic acid vaccines. Semin Immunol 2013; 25:152-9.
Sahin U, Kariko K, Tuereci O. mRNA-based therapeutics - developing a new class of drugs. Nat Rev Drug Discov 2014;13:759-80.
Smith ML, Corbo T, Bernales J, Lindbo JA, Pogue GP, Palmer KE, et al. Assembly of trans-encapsidated recombinant viral vectors engineered from Tobacco mosaic virus and Semliki Forest virus and their evaluation as immunogens. Virology 2007;358:321-33.
Azizgolshani O, Garmann RF, Cadena-Nava R, Knobler CM, Gelbart WM. Reconstituted plant viral capsids can release genes to mammalian cells. Virology 2013;441: 12-7.
Shin HI, Kim IC, Cho TJ. Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA. BMB Rep 2008;41:739-44.
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Flock House Virus RNA1 with a Long Heterologous Sequence at the 3’-end Can Replicate in Mammalian Cells and Mediate Reporter Gene Expression
Journal of Microbiology and Biotechnology
2019 .01
-
Isolation and Molecular Characterization of Feline Herpesvirus 1 from Naturally Infected Korean Cats
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .12
-
N-terminal Extension of Coat Protein of Turnip Yellow Mosaic Virus has Variable Effects on Replication, RNA Packaging, and Virion Assembly Depending on the Inserted Sequence
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2016 .03
-
Cysteine-Added Mutants of Turnip Yellow Mosaic Virus
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2018 .12
-
The Crucial Role of Chloroplast-Related Proteins in Viral Genome Replication and Host Defense against Positive-Sense Single-Stranded RNA Viruses
The Plant Pathology Journal
2023 .02
-
Combining the Power of Advanced Proteome-wide Sample Preparation Methods and Mass Spectrometry for defining the RNA-Protein Interactions
Mass Spectrometry Letters
2022 .12
-
식물 RNA 바이러스의 진화와 병저항성 극복 기작
Research in Plant Disease(식물병연구)
2021 .12
-
Ovarian transcriptome profiles associated with sexual maturation in Pacific abalone (Haliotis discus hannai)
Genes & Genomics
2020 .01
-
Comparative analysis of the Down syndrome hippocampal non-coding RNA transcriptomes using a mouse model
Genes & Genomics
2020 .01
-
Determinants of RNA Function
한국미생물학회 학술대회논문집
2022 .10
-
Incredible RNA: Dual Functions of Coding and Noncoding
Molecules and Cells
2016 .05
-
Circular RNAs in and out of Cells: Therapeutic Usages of Circular RNAs
Molecules and Cells
2023 .01
-
Heterogeneous Sequences of Brain Cytoplasmic 200 RNA Formed by Multiple Adenine Nucleotide Insertions
Molecules and Cells
2019 .01
-
Generation and Retention of Defective RNA3 from Cucumber Mosaic Virus and Relevance of the 2b Protein to Generation of the Subviral RNA
The Plant Pathology Journal
2023 .12
-
순무황화모자이크바이러스에 감염된 배추 세포의 엽록체에서의 미세 구조 특성 및 바이러스 증식 장소
Research in Plant Disease(식물병연구)
2024 .12
-
Application of small RNA for diagnosis and prognosis
한국자기학회 학술연구발표회 논문개요집
2024 .05
-
Emerging Roles of RNA-Binding Proteins in Plant Growth, Development, and Stress Responses
Molecules and Cells
2016 .03
-
The Detection of Plant Viruses in Korean Ginseng (Panax ginseng) through RNA Sequencing
The Plant Pathology Journal
2020 .12
-
High Resolution Mapping of RNA-Protein Interactions & SARS-CoV-2 RNA interactome
한국분석과학회 학술대회
2021 .05
-
The Characteristics of RNA Vaccine
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2016 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-475-001006255