지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
The formation of brown colonies due to phenol oxidase activity on classic agar media containing natural material extracts of Helianthus annuus or on medium containing L-3,4-dihydroxyphenylalanine has been used to identify Cryptococcus species complex. In this study, various natural materials were used to develop a modified medium and to identify five major serotypes of Cryptococcus species complex. Serotypes A, D, and A/D were pigmented on medium using Perilla frutescens var. japonica Hara (PerJ agar) after a three-day incubation. Serotypes B and C were pigmented on PerJ agar after four- and five-day incubations, respectively. Growth time and pigmentation of the five serotypes occurred more rapidly on PerJ agar than on the other media. In addition, colony morphology, size, and pigmentation were specific by serotype. In conclusion, PerJ agar should be used in clinic settings to identify Cryptococcus species complex and its serotypes rapidly.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (16)
참고문헌 신청
Casadevall A, Rosas AL, Nosanchuk JD. Melanin and virulence in Cryptococcus neoformans. Current Opinion in Microbiology. 2003. 3: 354-358.
Chae HS, Park GN, Kim SH, Jo HJ, Kim JT, Jeoung HY, An DJ, Kim NH, Shin BW, Kang YI, Chang KS. Rapid direct identification of Cryptococcus neoformans from pigeon droppings by nested PCR using CNLAC1 gene. Poultry Science. 2012. 91: 1983-1989.
Chae HS, Jang GE, Kim NH, Son HR, Lee JH, Kim SH, Park GN, Jo HJ, Kim JT, Chang KS. Classification of Cryptococcus neoformans and yeast-like fungus isolates from pigeon droppings by colony phenotyping and ITS genotyping and their seasonal variations in Korea. Avian Diseases. 2012. 56: 58-64.
Eisenman HC, Chow SK, Tse KK, McClelland EE, Casadeval A. The effect of L-DOPA on Cryptococcus neoformans growth and gene expression. Virulence. 2011. 2: 329-336.
Garcia-Rivera J, Eisenman HC, Nosanchuk JD, Aisen P, Zaragoza O, Moadel T, Dadachova E, Casadevall A. Comparative analysis of Cryptococcus neoformans acid-resistant particles generated from pigmented cells grown in different laccase substrates. Fungal Genetics and Biology. 2005. 42: 989-998.
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UCI(KEPA) : I410-ECN-0101-2018-510-001017567