염증성 사이토카인의 발현 억제를 통한 홍해삼 추출물의 항염증효과 및 아토피 개선효과

전미지; 임은경; 김가연; 이승제; 정인철; 김상용; 김영민

doi:10.7841/ksbbj.2017.32.4.335

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자료유형: 학술저널

저자정보: 전미지 (한남대학교) 임은경 (한남대학교) 김가연 (한남대학교) 이승제 (한남대학교) 정인철 (신한에코) 김상용 (신안산대학교) 김영민 (한남대학교)

저널정보: 한국생물공학회 KSBB Journal KSBB Journal Vol.32 No.4(Wn.163)

발행연도: 2017.12

수록면: 335 - 341 (7page)

DOI: 10.7841/ksbbj.2017.32.4.335

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The red sea cucumber (RSC), Apostichopus japonicus, is a species of sea cucumber in the family Stichopodidae. It is widely distributed in China, Japan and Korea. Tumor necrosis factor-α (TNF-α) and Interferon (IFN-γ) induce inflammatory cytokines such as Thymus and activation-regulated chemokine (TARC/CCL17), Cutaneous T-cellattracting chemokine (CTACK/CCL27), interleukin-1β (IL-1β), IL-6 and IL-8. These cytokines are increased in atopic dermatitis condition. In this study, we investigated the antiinflammatory and skin regeneration effects by RCS. To investigate cytotoxic effects in HaCaT cells, we performed MTT assay after treatment with RSC extracted by 70% EtOH. As a result, RSC extracts did not have cytotoxic effect in HaCaT cells. Also, mRNA expression levels of CCL17, CCL27, IL-β, IL-6 and IL-8 genes induced by TNF-α/IFN-γ were decreased by RSC extracts. Moreover, we confirmed the release of CCL17 and CCL27 protein through ELISA assay. Wound healing assay and cell cycle assay were performed to identify cell regeneration effect of RSC extracts. These results demonstrated the prevention and treatment effects of RSC on skin inflammatory diseases such as atopic dermatitis.

#red sea cucumber #keratinocyte #atopic dermatitis #anti-inflammatory

참고문헌 (26)

참고문헌 신청

De Benedetto, A., A. Kubo, and L. A. Beck (2012) Skin barrier disruption: a requirement for allergen sensitization. J. Invest. Dermatol. 132: 949-963. Masuoka, M., H. Shiraishi, S. Ohta, S. Suzuki, K. Arima, S. Aoki, S. Toda, N. Inagaki, Y. Kurihara, S. Hayashida, S. Takeuchi, K. Koike, J. Ono, H. Noshiro, M. Furue, S. J. Conway, Y. Narisawa, and K. Izuhara (2012) Periostin promotes chronic allergic inflammation in response to Th2 cytokines. J. Clin. Invest. 122: 2590-2600. Lee, H. J. and Y. C. Chang (2012) Suppression of TNF-α-induced inflammation by extract from different parts of Moringa in HaCaT cells. J. Life Sci. 22: 1254-1260. Hudson, T. J. (2006) Skin barrier function and allergic risk. Nature Genetics 38: 399-400. Chin, S. P. and D. H. Lee (2014) Epidermal structure and skin barrier. J. Skin Barrier Res. 16: 5-11.

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