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자연과학
공학
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예술체육학
복합학
DBpia AI
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커뮤니티
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학술저널
Full-text AI
오류 신고하기해당 페이지 내 제목·저자·목차·페이지정보가 잘못된 경우 알려주세요!
초록·키워드
Background: Panax notoginseng leaves (PNL) exhibit extensive activities, but few analytical methods have been established to exclusively determine the dammarane triterpene saponins in PNL.
Methods: Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOF MS) and HPLC-UV methods were developed for the qualitative and quantitative analysis of ginsenosides in PNL, respectively.
Results: Extraction conditions, including solvents and extraction methods, were optimized, which showed that ginsenosides Rc and Rb3, the main components of PNL, are transformed to notoginsenosides Fe and Fd, respectively, in the presence of water, by removing a glucose residue from position C-3 via possible enzymatic hydrolysis. A total of 57 saponins were identified in the methanolic extract of PNL by UPLC/QTOF MS. Among them, 19 components were unambiguously characterized by their reference substances. Additionally, seven saponins of PNLdginsenosides Rb1, Rc, Rb2, and Rb3, and notoginsenosides Fc, Fe, and Fddwere quantified using the HPLC-UV method after extraction with methanol. The separation of analytes, particularly the separation of notoginsenoside Fc and ginsenoside Rc, was achieved on a Zorbax ODS C8 column at a temperature of 35℃. This developed HPLC-UV method provides an adequate linearity (r²> 0.999), repeatability (relative standard deviation, RSD < 2.98%), and inter- and intraday variations (RSD < 4.40%) with recovery (98.7e106.1%) of seven saponins concerned. This validated method was also conducted to determine seven components in 10 batches of PNL.
Conclusion: These findings are beneficial to the quality control of PNL and its relevant products.
Methods: Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOF MS) and HPLC-UV methods were developed for the qualitative and quantitative analysis of ginsenosides in PNL, respectively.
Results: Extraction conditions, including solvents and extraction methods, were optimized, which showed that ginsenosides Rc and Rb3, the main components of PNL, are transformed to notoginsenosides Fe and Fd, respectively, in the presence of water, by removing a glucose residue from position C-3 via possible enzymatic hydrolysis. A total of 57 saponins were identified in the methanolic extract of PNL by UPLC/QTOF MS. Among them, 19 components were unambiguously characterized by their reference substances. Additionally, seven saponins of PNLdginsenosides Rb1, Rc, Rb2, and Rb3, and notoginsenosides Fc, Fe, and Fddwere quantified using the HPLC-UV method after extraction with methanol. The separation of analytes, particularly the separation of notoginsenoside Fc and ginsenoside Rc, was achieved on a Zorbax ODS C8 column at a temperature of 35℃. This developed HPLC-UV method provides an adequate linearity (r²> 0.999), repeatability (relative standard deviation, RSD < 2.98%), and inter- and intraday variations (RSD < 4.40%) with recovery (98.7e106.1%) of seven saponins concerned. This validated method was also conducted to determine seven components in 10 batches of PNL.
Conclusion: These findings are beneficial to the quality control of PNL and its relevant products.
본문·목차
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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