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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 409 - 418 (10page)
이용수
초록· 키워드
오류제보하기
Acanthamoeba spp. are free-living protozoa that are opportunistic pathogens for humans. Cysteine proteases of Acanthamoeba have been partially characterized, but their biochemical and functional properties are not clearly understood yet. In this study, we isolated a gene encoding cysteine protease of A. castellanii (AcCP) and its biochemical and functional properties were analyzed. Sequence analysis of AcCP suggests that this enzyme is a typical cathepsin L family cysteine protease, which shares similar structural characteristics with other cathepsin L-like enzymes. The recombinant AcCP showed enzymatic activity in acidic conditions with an optimum at pH 4.0. The recombinant enzyme effectively hydrolyzed human proteins including hemoglobin, albumin, immunoglobuins A and G, and fibronectin at acidic pH. AcCP mainly localized in lysosomal compartment and its expression was observed in both trophozoites and cysts. AcCP was also identified in cultured medium of A. castellanii. Considering to lysosomal localization, secretion or release by trophozoites and continuous expression in trophozoites and cysts, the enzyme could be a multifunctional enzyme that plays important biological functions for nutrition, development and pathogenicity of A. castellanii. These results also imply that AcCP can be a promising target for development of chemotherapeutic drug for Acanthamoeba infections.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (38)
참고문헌 신청
[학술지(정기간행물)] - Khan NA / 2009 / Acanthamoeba affects the integrity of human brain microvascular endothelial cells and degrades the tight junction proteins / Int J Parasitol 39 : 1611 ~ 1616
[학술지(정기간행물)] - Marciano-Cabral F / 2003 / Acanthamoeba spp. as agents of disease in humans / Clin Microbiol Rev 16 : 273 ~ 307
[학술지(정기간행물)] - Bowers B / 1977 / Comparison of pinocytosis and phagocytosis in Acanthamoeba castellanii / Exp Cell Res 110 : 409 ~ 417
[학술지(정기간행물)] - Bowers B / 1983 / Acanthamoeba discriminates internally between digestible and indigestible particles / J Cell Biol 97 : 317 ~ 322
[학술지(정기간행물)] - Lloyd D / 2001 / Encystation in Acanthamoeba castellanii: development of biocide resistance / J Eukaryot Microbiol 48 : 11 ~ 16
[학술지(정기간행물)] - Marciano-Cabral F / 2003 / Acanthamoeba spp. as agents of disease in humans / Clin Microbiol Rev 16 : 273 ~ 307
[학술지(정기간행물)] - Bowers B / 1977 / Comparison of pinocytosis and phagocytosis in Acanthamoeba castellanii / Exp Cell Res 110 : 409 ~ 417
[학술지(정기간행물)] - Bowers B / 1983 / Acanthamoeba discriminates internally between digestible and indigestible particles / J Cell Biol 97 : 317 ~ 322
[학술지(정기간행물)] - Lloyd D / 2001 / Encystation in Acanthamoeba castellanii: development of biocide resistance / J Eukaryot Microbiol 48 : 11 ~ 16
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