지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 419 - 428 (10page)
이용수
초록· 키워드
오류제보하기
Background: Despite the large number of studies on ginseng, pharmacological activities of ginseng seed oil (GSO) have not been established. GSO is rich in unsaturated fatty acids, mostly oleic and linoleic acids. Unsaturated fatty acids are known to exert a therapeutic effect in nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the protective effect and underlying mechanisms of GSO against NAFLD using in vitro and in vivo models.
Methods: In vitro lipid accumulation was induced by free fatty acid mixture in HepG2 cells and by 3 wk of high fat diet (HFD)-feeding in SpragueeDawley rats prior to hepatocyte isolation. The effects of GSO against diet-induced hepatic steatosis were further examined in C57BL/6J mice fed a HFD for 12 wk. Results: Oil Red O staining and intracellular triglyceride levels showed marked accumulation of lipid droplets in both HepG2 cells and rat hepatocytes, and these were attenuated by GSO treatment. In HFDfed mice, GSO improved HFD-induced dyslipidemia and hepatic insulin resistance. Increased hepatic lipid contents were observed in HFD-fed mice and it was lowered in GSO (500 mg/kg)-treated mice by 26.4% which was evident in histological analysis. Pathway analysis of hepatic global gene expression indicated that GSO increased the expression of genes associated with β-oxidation (Ppara, Ppargc1a, Sirt1, and Cpt1a) and decreased the expression of lipogenic genes (Srebf1 and Mlxipl), and these were confirmed with reverse transcription and quantitative polymerase-chain reaction.
Conclusion: These findings suggest that GSO has a beneficial effect on NAFLD through the suppression of lipogenesis and stimulation of fatty acid degradation pathway.
Methods: In vitro lipid accumulation was induced by free fatty acid mixture in HepG2 cells and by 3 wk of high fat diet (HFD)-feeding in SpragueeDawley rats prior to hepatocyte isolation. The effects of GSO against diet-induced hepatic steatosis were further examined in C57BL/6J mice fed a HFD for 12 wk. Results: Oil Red O staining and intracellular triglyceride levels showed marked accumulation of lipid droplets in both HepG2 cells and rat hepatocytes, and these were attenuated by GSO treatment. In HFDfed mice, GSO improved HFD-induced dyslipidemia and hepatic insulin resistance. Increased hepatic lipid contents were observed in HFD-fed mice and it was lowered in GSO (500 mg/kg)-treated mice by 26.4% which was evident in histological analysis. Pathway analysis of hepatic global gene expression indicated that GSO increased the expression of genes associated with β-oxidation (Ppara, Ppargc1a, Sirt1, and Cpt1a) and decreased the expression of lipogenic genes (Srebf1 and Mlxipl), and these were confirmed with reverse transcription and quantitative polymerase-chain reaction.
Conclusion: These findings suggest that GSO has a beneficial effect on NAFLD through the suppression of lipogenesis and stimulation of fatty acid degradation pathway.
목차
abstract
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (34)
참고문헌 신청
[학술지(정기간행물)] - Seo E / 2015 / Ginseng berry extract supplementation improves age-related decline of insulin signaling in mice / Nutrients 7 : 3038 ~ 3053
[학술지(정기간행물)] - Kim WK / 2013 / Woundhealing effect of ginsenoside Rd from leaves of Panax ginseng via cyclic AMPdependent protein kinase pathway / Eur J Pharmacol 702 : 285 ~ 293
[학술지(정기간행물)] - Yang BR / 2016 / Amelioration of acute myocardial infarction by saponins from flower buds of Panax notoginseng via pro-angiogenesis and anti-apoptosis / J Ethnopharmacol 181 : 50 ~ 58
[학술지(정기간행물)] - Wang Y / 2012 / Effects of Panax notoginseng flower extract on the TGF-beta/Smad signal transduction pathway in heart remodeling of human chymase transgenic mice / Mol Med Rep 5 : 1443 ~ 1448
[학술지(정기간행물)] - Myung-Hee Lee / 2013 / Quality characteristics of ginseng seed oil treated by different extraction methods / Journal of Ginseng Research 37 (4) : 468 ~ 474
[학술지(정기간행물)] - Kim WK / 2013 / Woundhealing effect of ginsenoside Rd from leaves of Panax ginseng via cyclic AMPdependent protein kinase pathway / Eur J Pharmacol 702 : 285 ~ 293
[학술지(정기간행물)] - Yang BR / 2016 / Amelioration of acute myocardial infarction by saponins from flower buds of Panax notoginseng via pro-angiogenesis and anti-apoptosis / J Ethnopharmacol 181 : 50 ~ 58
[학술지(정기간행물)] - Wang Y / 2012 / Effects of Panax notoginseng flower extract on the TGF-beta/Smad signal transduction pathway in heart remodeling of human chymase transgenic mice / Mol Med Rep 5 : 1443 ~ 1448
[학술지(정기간행물)] - Myung-Hee Lee / 2013 / Quality characteristics of ginseng seed oil treated by different extraction methods / Journal of Ginseng Research 37 (4) : 468 ~ 474
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
용담화 에탄올 추출물의 항노화 작용 연구
한방안이비인후피부과학회지
2015 .01
-
용담화 에탄올 추출물의 항염증 효과에 관한 연구
한방안이비인후피부과학회지
2015 .01
-
Artemisia annua extract ameliorates high-fat diet-induced fatty liver by activating AMPK
Journal of Biomedical and Translational Research
2020 .01
-
Silkworm attenuates non-alcoholic fatty liver disease in free fatty acid-induced HepG2 hepatocytes and in high fat diet-fed mice
한국실험동물학회 학술발표대회 논문집
2020 .07
-
Rho-Kinase as a Therapeutic Target for Nonalcoholic Fatty Liver Diseases
Diabetes and Metabolism Journal
2021 .09
-
Theracurmin (Highly Bioavailable Curcumin) Prevents High Fat Diet-Induced Hepatic Steatosis Development in Mice
Toxicological Research
2019 .10
-
Histological Analysis of Hepatic Steatosis, Inflammation, and Fibrosis in Ascorbic Acid-Treated Ovariectomized Mice
대한의생명과학회지
2022 .06
-
Ameliorative effects of black ginseng on nonalcoholic fatty liver disease in free fatty acid-induced HepG2 cells and high-fat/high-fructose diet-fed mice
Journal of Ginseng Research
2020 .03
-
Ginsenosides Rc, as a novel SIRT6 activator, protects mice against high fat diet induced NAFLD
Journal of Ginseng Research
2023 .05
-
산사의 NAFLD 보호 효과 및 그 작용기전에 관한 연구
대한본초학회지(본초분과학회지)
2018 .01
-
Endogenous catalase delays high-fat diet-induced liver injury in mice
The Korean Journal of Physiology & Pharmacology
2017 .05
-
External electrical stimulation suppresses hepatic lipid accumulation by modulating lipid metabolism in non-alcoholic fatty liver animal models
한국실험동물학회 학술발표대회 논문집
2022 .07
-
Inhibition of Serotonin Synthesis Induces Negative Hepatic Lipid Balance
Diabetes and Metabolism Journal
2018 .01
-
Integrative Omics Reveals Metabolic and Transcriptomic Alteration of Nonalcoholic Fatty Liver Disease in Catalase Knockout Mice
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Ginseng-plus-Bai-Hu-Tang ameliorates diet-induced obesity, hepatic steatosis, and insulin resistance in mice
Journal of Ginseng Research
2020 .03
-
비알코올성 지방간 질환과 소포체 스트레스
대한내과학회지
2021 .04
-
RIG -I regulates metabolism in a high fat diet-induced nonalcoholic fatty liver disease mouse model
한국실험동물학회 학술발표대회 논문집
2021 .01
-
Hepatic MIR20B promotes nonalcoholic fatty liver disease by suppressing PPARA
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Downregulation of GNAI3 Promotes the Pathogenesis of Methionine/Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease
Gut and Liver
2020 .01
-
Non-alcoholic Fatty Liver Disease (NAFLD) and Significant Hepatic Fibrosis Defined by Non-invasive Assessment in Patients with Type 2 Diabetes
Asian Pacific journal of cancer prevention : APJCP
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0