지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 38 - 48 (11page)
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초록· 키워드
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Background: Interspecific ginseng hybrid, Panax ginseng × Panax quenquifolius (Pgq) has vigorous growth and produces larger roots than its parents. However, F₁ progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid.
Methods: Murashige and Skoog (MS) medium with different concentrations (0, 2, 4, and 6 mg/L) of 2,4- dichlorophenoxyacetic acid (2,4-D) was used for callus induction and somatic embryogenesis (SE). The embryos, after culturing on GA₃ supplemented medium, were transferred to hormone free 1/2 Schenk and Hildebrandt (SH) medium. The developed taproots with dormant buds were treated with GA₃ to break the bud dormancy, and transferred to soil. Hybrid Pgq plants were verified by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses and by LC-IT-TOF-MS.
Results: We conducted a comparative study of somatic embryogenesis (SE) in Pgq and its parents, and attempted to establish the soil transfer of in vitro propagated Pgq tap roots. The Pgq explants showed higher rate of embryogenesis (~56% at 2 mg/L 2,4-D concentration) as well as higher number of embryos per explants (~7 at the same 2,4-D concentration) compared to its either parents. The germinated embryos, after culturing on GA₃ supplemented medium, were transferred to hormone free 1/2 SH medium to support the continued growth and kept until nutrient depletion induced senescence (NuDIS) of leaf defoliation occurred (4 months). By that time, thickened tap roots with well-developed lateral roots and dormant buds were obtained. All Pgq tap roots pretreated with 20 mg/L GA₃ for at least a week produced new shoots after soil transfer. We selected the discriminatory RAPD and ISSR markers to find the interspecific ginseng hybrid among its parents. The F₁ hybrid (Pgq) contained species specific 2 ginsenosides (ginsenoside Rf in P. ginseng and pseudoginsenosides F<SUB>11</SUB> in P. quinquefolius), and higher amount of other ginsenosides than its parents.
Conclusion: Micropropagation of interspecific hybrid ginseng can give an opportunity for continuous production of plants.
Methods: Murashige and Skoog (MS) medium with different concentrations (0, 2, 4, and 6 mg/L) of 2,4- dichlorophenoxyacetic acid (2,4-D) was used for callus induction and somatic embryogenesis (SE). The embryos, after culturing on GA₃ supplemented medium, were transferred to hormone free 1/2 Schenk and Hildebrandt (SH) medium. The developed taproots with dormant buds were treated with GA₃ to break the bud dormancy, and transferred to soil. Hybrid Pgq plants were verified by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses and by LC-IT-TOF-MS.
Results: We conducted a comparative study of somatic embryogenesis (SE) in Pgq and its parents, and attempted to establish the soil transfer of in vitro propagated Pgq tap roots. The Pgq explants showed higher rate of embryogenesis (~56% at 2 mg/L 2,4-D concentration) as well as higher number of embryos per explants (~7 at the same 2,4-D concentration) compared to its either parents. The germinated embryos, after culturing on GA₃ supplemented medium, were transferred to hormone free 1/2 SH medium to support the continued growth and kept until nutrient depletion induced senescence (NuDIS) of leaf defoliation occurred (4 months). By that time, thickened tap roots with well-developed lateral roots and dormant buds were obtained. All Pgq tap roots pretreated with 20 mg/L GA₃ for at least a week produced new shoots after soil transfer. We selected the discriminatory RAPD and ISSR markers to find the interspecific ginseng hybrid among its parents. The F₁ hybrid (Pgq) contained species specific 2 ginsenosides (ginsenoside Rf in P. ginseng and pseudoginsenosides F<SUB>11</SUB> in P. quinquefolius), and higher amount of other ginsenosides than its parents.
Conclusion: Micropropagation of interspecific hybrid ginseng can give an opportunity for continuous production of plants.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (64)
참고문헌 신청
Sharma SK / 2009 / A new species of Panax L. (Araliaceae) from Sikkim Himalaya / India Syst Bot 34:434~438
Chan TWD / 2000 / Differentiation and authentication of Panax ginseng, Panax quinquefolius, and ginseng products by using HPLC/MS / Anal Chem 72:1281~1287
Choi KT / 2008 / Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer / Acta Pharmacol Sin 29:1109~1118
Kim JH / 2013 / Diversity and evolution of major Panax species revealed by scanning the entire chloroplast intergenic spacer sequences / Genet Resour Crop Evol 60:413~425
Christensen LP / 2009 / Ginsenosides chemistry, biosynthesis, analysis, and potential health effects / Adv Food Nutr Res 55:1~99
Chan TWD / 2000 / Differentiation and authentication of Panax ginseng, Panax quinquefolius, and ginseng products by using HPLC/MS / Anal Chem 72:1281~1287
Choi KT / 2008 / Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer / Acta Pharmacol Sin 29:1109~1118
Kim JH / 2013 / Diversity and evolution of major Panax species revealed by scanning the entire chloroplast intergenic spacer sequences / Genet Resour Crop Evol 60:413~425
Christensen LP / 2009 / Ginsenosides chemistry, biosynthesis, analysis, and potential health effects / Adv Food Nutr Res 55:1~99
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UCI(KEPA) : I410-ECN-0101-2019-524-000425292