지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.8
- 수록면
- 277 - 285 (9page)
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초록· 키워드
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In this study, Expressed Sequence Tag-Simple Sequence Repeat(EST-SSR) analyses were used to clarify the genetic polymorphisms among Korean ginseng cultivars and breeding lines and to classify them into distinct genetic groups. Polymorphic and reproducible bands were produced by 14 primers out of total 30 primers used in this study. Fourteen EST-SSR loci generated a total of 123 bands. Amplified PCR products showed the highly reproducible banding patterns at 110~920 bp. The number of amplified bands for each EST-SSR primers ranged from 2 to 19 with a mean of 8.8 bands. P26 and P35 primers showed 13 and 12 banding patterns, respectively. The number of alleles for each EST-SSR locus ranged from 1.67 to 2.00 with a mean of 1.878 alleles. P34 and P60 primers showed the highest and the lowest genetic polymorphism, respectively. Cluster analysis based on genetic similarity estimated by EST-SSR markers classified Korean cultivars and breeding lines into 4 groups. Group included Gopoong and Chunpoong and 9 breeding lines (55%), group included 2 breeding lines (10%), group included 3 breeding lines (15%),group included Gum poong and 3 breeding lines (20%). Consequentlym, the EST-SSR marker developed in this study may prove useful for the evaluation of genetic diversity and diffeentiation of Korean ginseng cultivars and breeding lines.
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참고문헌 (37)
참고문헌 신청
방경환 / 2011 / SSR 마커를 이용한 고려인삼 품종 판별기술 개발 / 한국약용작물학회지 19(3):185~190
방경환 / 2011 / SSR 마커를 이용한 국내산 인삼 품종 및 국외 수집종의 유전적 다양성 분석 / 한국약용작물학회지 19(5):347~353
Bang KH / 2010 / Construction of genomic DNA library of Korean ginseng (Panax ginseng C. A. Meyer) and development of sequence-tagged sites / Biological and Pharmaceutical Bulletin 33:1579~1588
Botstein D / 1980 / Construction of a genetic linkage map in man using restriction fragment length polymorphism / American Journal of Human Genetics 32:314~331
Chao S / 1989 / RFLP-based genetic maps of wheat homoeologous group-7 chromosomes / Theoretical and Applied Genetics 78:495~495
방경환 / 2011 / SSR 마커를 이용한 국내산 인삼 품종 및 국외 수집종의 유전적 다양성 분석 / 한국약용작물학회지 19(5):347~353
Bang KH / 2010 / Construction of genomic DNA library of Korean ginseng (Panax ginseng C. A. Meyer) and development of sequence-tagged sites / Biological and Pharmaceutical Bulletin 33:1579~1588
Botstein D / 1980 / Construction of a genetic linkage map in man using restriction fragment length polymorphism / American Journal of Human Genetics 32:314~331
Chao S / 1989 / RFLP-based genetic maps of wheat homoeologous group-7 chromosomes / Theoretical and Applied Genetics 78:495~495
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UCI(KEPA) : I410-ECN-0101-2019-480-000588908