지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.2
- 수록면
- 26 - 31 (6page)
- DOI
- 10.7783/KJMCS.2018.26.1.26
이용수
초록· 키워드
오류제보하기
Background: Angelica gigas, A. sinensis, and A. acutiloba are commercially important in the herbal medicine market, and among them, A. gigas has the highest economic value and price. However, their similar morphological traits are often used for fraud. Despite their importance in herbal medicine, recognition of the differences between Angelica species is currently inadequate.
Methods and Results: A multiplex polymerase chain reaction (PCR) method was developed for direct detection and identification of A. gigas, A. sinensis, and A. acutiloba. The gene for the distinction of species was targeted at ITS in the nucleus and trnC-petN gene in chloroplasts. The optimized multiplex PCR in the present study utilized each Angelica species-specific primer pairs. Each primer pair yielded products of 229 base pairs (bp) for A. gigas, 53 bp for A. sinensis, 170 bp for A. acutiloba. Additionally non-specific PCR products were not detected in similar species by species-specific primers.
Conclusions: In the present study, a multiplex-PCR assay, successfully assessed the authenticity of Angelica species (A. gigas, A. sinensis, and A. acutiloba). and whole genome amplification (WGA) was performed after DNA extraction to identify, the species in the product. The detection method of raw materials developed in the present study could be applied to herbal medicine and health functional food management.
Methods and Results: A multiplex polymerase chain reaction (PCR) method was developed for direct detection and identification of A. gigas, A. sinensis, and A. acutiloba. The gene for the distinction of species was targeted at ITS in the nucleus and trnC-petN gene in chloroplasts. The optimized multiplex PCR in the present study utilized each Angelica species-specific primer pairs. Each primer pair yielded products of 229 base pairs (bp) for A. gigas, 53 bp for A. sinensis, 170 bp for A. acutiloba. Additionally non-specific PCR products were not detected in similar species by species-specific primers.
Conclusions: In the present study, a multiplex-PCR assay, successfully assessed the authenticity of Angelica species (A. gigas, A. sinensis, and A. acutiloba). and whole genome amplification (WGA) was performed after DNA extraction to identify, the species in the product. The detection method of raw materials developed in the present study could be applied to herbal medicine and health functional food management.
목차
ABSTRACT
서언
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (18)
참고문헌 신청
Arbona V / 2009 / Plant phenotype demarcation using non-targeted LC-MS and GC-MS metabolite profiling / Journal of Agricultural and Food Chemistry 57:7338~7347
조창희 / 2002 / 근적외선분광법, 전자코 및 엑스 선형광법을 이용한 당귀의 기원판별법 비교 연구 / 약 학 회 지 46(3):161~167
방재옥 / 2005 / Angelica속 식물 7종의 세포유전학적 분석 / 한국약용작물학회지 13(3):118~121
Choo BK / 2009 / Development of SCAR markers for the discrimination of three species of medicinal plants, Angelica decursiva(Peucedanum decursivum), Peucedanum praeruptorum and Anthricus sylvestris, based on the internal transcribed spacer(ITS) sequence and random amplified polymorphic DNA(RAPD) / Biological and Pharmaceutical Bulletin 32:24~30
Guo YL / 2005 / Molecular phylogeny of Oryzeae (Poaceae) based on DNA sequences from chloroplast, mitochondrial, and nuclear genomes / American Journal of Botany 92:1548~1558
조창희 / 2002 / 근적외선분광법, 전자코 및 엑스 선형광법을 이용한 당귀의 기원판별법 비교 연구 / 약 학 회 지 46(3):161~167
방재옥 / 2005 / Angelica속 식물 7종의 세포유전학적 분석 / 한국약용작물학회지 13(3):118~121
Choo BK / 2009 / Development of SCAR markers for the discrimination of three species of medicinal plants, Angelica decursiva(Peucedanum decursivum), Peucedanum praeruptorum and Anthricus sylvestris, based on the internal transcribed spacer(ITS) sequence and random amplified polymorphic DNA(RAPD) / Biological and Pharmaceutical Bulletin 32:24~30
Guo YL / 2005 / Molecular phylogeny of Oryzeae (Poaceae) based on DNA sequences from chloroplast, mitochondrial, and nuclear genomes / American Journal of Botany 92:1548~1558
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