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A recent report showed that nuclear factor of activated T cell (NFATc) 1 is a member of the NFAT family and is strictly implicated osteoblast differentiation and bone formation. Furthermore, the precise expression and function of NFATc1 in periodontal tissue remains unclear. Therefore, the purpose of this study was to investigate the function of NFATc1 in osteoblastic differentiation, and the underlying mechanism regulating periodontal regeneration in human periodontal ligament cells (hPDLCs). NFATc1 messenger RNA (mRNA) and protein levels were accessed by reverse transcription-polymerase chain reaction (RT-PCR) and western blot assay, respectively. Cell proliferation determined using MTT assay. Differentiation was evaluated by alkaline phosphatase activity and formation of calcium nodule with alizarin red S staining. The mRNA expression of osteoblastic differentiation related genes were examined by RT-PCR. Marked upregulation of NFATc1 mRNA and protein was observed in cells grown in osteogenic medium (OS). NFATc1 transactivation was detected in hPDLCs that had been incubated in OS for 14 days. Treatment with 10 μM cyclosporine A (CsA), a known calcineurin inhibitor, reduced the proliferation of hPDLCs, while 5 μM CsA had no effect. Inhibition of the calcineurin/NFATc1 pathway by CsA, attenuated OS-induced osteoblastic differentiation in hPDLCs. In summary, this study demonstrates for the first time that NFATc1 plays a key role in osteoblastic differentiation of hPDLCsand activation of NFATc1 could provide a novel mechanism for periodontal bone regeneration.
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참고문헌 (26)
참고문헌 신청
Bartold PM / 2000 / Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology / Periodontol 24 : 253 ~ 269
Beals CR / 1997 / Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction / Genes Dev 11 : 824 ~ 834
Beertsen W / 1997 / The periodontal ligament:a unique, multifunctional connective tissue / Periodontol 2000 13 : 20 ~ 40
Chang CP / 2004 / A field of myocardial-endocardial NFAT signaling underlies heart valve morphogenesis / Cell 118 : 649 ~ 663
Crabtree GR / 2002 / NFAT signaling: choreographing the social lives of cells / Cell Suppl 109 : S67 ~ S79
Beals CR / 1997 / Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction / Genes Dev 11 : 824 ~ 834
Beertsen W / 1997 / The periodontal ligament:a unique, multifunctional connective tissue / Periodontol 2000 13 : 20 ~ 40
Chang CP / 2004 / A field of myocardial-endocardial NFAT signaling underlies heart valve morphogenesis / Cell 118 : 649 ~ 663
Crabtree GR / 2002 / NFAT signaling: choreographing the social lives of cells / Cell Suppl 109 : S67 ~ S79
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