Involvement of Nuclear Factor Kappa B in High-Fat Diet-Related Pancreatic  Fibrosis in Rats

Ming-Xian Yan; Hong-Bo Ren; Yi Kou; Min Meng; Yan-Qing Li

추천

검색

자료유형: 학술저널

저자정보: Ming-Xian Yan Hong-Bo Ren Yi Kou Min Meng Yan-Qing Li

저널정보: 거트앤리버 발행위원회 Gut and Liver Gut and Liver 제6권 제3호

발행연도: 2012.1

수록면: 381 - 387 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background/Aims: High-fat diets contribute to pancreatic fibrogenesis, but the pathogenesis remains unclear. This study investigated the role of nuclear factor kappa B (NF-κB)in high-fat diet-induced pancreatic fi brosis in rats. Methods:Male Wistar rats were fed a high-fat diet or standard normal chow for 20 weeks. Pancreatic fibrosis was determined by Sirius red staining. Immunohistochemical staining, reverse transcription-polymerase chain reaction and Western blotting were used to identify NF-κB-associated genes or protein expressions. Results: Infl ammation, fat deposition, pancreatic stellate cell activation and fi brosis were observed in the pancreases of the high-fat diet group. NF-κB subunit p65 (NF-κB/p65) expression was localized to the nucleus, and intercellular adhesion molecule 1 (ICAM-1) was over-expressed. Pancreatic gene expression levels of NF-κB/p65, ICAM-1and tumor necrosis factor α were all elevated signifi cantly in rats fed a high-fat diet compared with control rats. Western blotting also revealed signifi cantly increased levels of ICAM-1and nuclear NF-κB/p65 in rats fed high-fat diets comparison with control rats. Conclusions: NF-κB is involved in high-fat diet-related pancreatic fi brosis.

#High-fat diet #Pancreatic fi brosis #NF-kappa B #Intercellular adhesion molecule 1 #Tumor necrosis factor-alpha

참고문헌 (23)

참고문헌 신청

Asaumi H / 2007 / Externally applied pressure activates pancreatic stellate cells through the generation of intracellular reactive oxygen species / Am J Physiol Gastrointest

Borst SE / 2005 / High-fat diet induces increased tissue expression of TNF-alpha / Life Sci 77 : 2156 ~ 2165

Chen F / 1999 / New insights into the role of nuclear factor-kappaB, a ubiquitous transcription factor in the initiation of diseases / Clin Chem 45 : 7 ~ 17

Czako L / 2007 / Hyperlipidemia induced by a cholesterol-rich diet aggravates necrotizing pancreatitis in rats / Eur J Pharmacol 572 : 74 ~ 81

Fraulob JC / 2010 / A mouse model of metabolic syndrome: insulin resistance, fatty liver and non-alcoholic fatty pancreas disease (NAFPD) in C57BL/6 mice fed a high fat diet