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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
대한한방소아과학회 대한한방소아과학회지 대한한방소아과학회지 제20권 제1호
발행연도
2006.1
수록면
219 - 240 (22page)

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Objective: This experimental study was designed to determine the anti-obestic effects of Bangpoongtongsungsankamibang(BTSK). Methods: In vitro, BTSK extracts of various concentration (50, 100, 200 ㎍/㎖) were added in 3T3-L1 cell. Adipocyte differentiation was measured by Oil Red O staining and morphological examination. The protein and mRNA expression of C/EBPα and PPARγ receptor was measured by western blot assay and RT-PCR. In vivo, BTSK extracts of various concentration (100, 200 ㎍/㎖) were orally administered to induced hyperlipidemic rats by poloxamer-407 for consecutive four weeks and serum triglyceride, total cholesterol were measured. This method applied to induced hyperlipidemic rats by triton WR-1339, too. Obesity induced rats by the high fat-diet for six weeks were orally administered BTSK extracts of various concentration (100, 200 ㎍/㎖) and serum triglyceride, total cholesterol, LDL-cholesterol, triglyceride, HDL- cholesterol, hydroxy radical, superoxide dismuatse activity were measured. Results : Ⅰ. In vitro 1. The 3T3-L1 cells’ differentiation was significantly decreased by BTSK. 2. The expression of C/EBPα and PPARγ was was significantly decreased by BTSK. Ⅱ. In vivo 1. BTSK significantly reduced serum triglyceride, total cholesterol contents in poloxamer-407 treated rat. 2. BTSK significantly reduced serum triglyceride contents in Triton WR-1339 treated rat. But Total cholesterol did not show a significant change. 3. BTSK significantly reduced body weight gain of rat and adipose tissue mass of rats and serum triglyceride, LDL-cholesterol contents and significantly increased HDL-cholesterol, HTR(HDL-cholesterol/Total-cholesterol) in rats with obesity induced by the high fat-diet. 4. BTSK reduced blood lipid peroxide, hydroxy radical and increased superoxide dismuatse(SOD) activity.

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