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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 573 - 578 (6page)
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초록· 키워드
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Several organophosphorus (OP) insecticides can selectively kill the silkworm maggot, Exorista sorbillans (Es) (Diptera: Tachinidae),while not obviously affecting the host (Bombyx mori) larvae,but the mechanism is not yet clear. In this study, the cDNA encoding an acetylcholinesterase (AChE) from the field Es was isolated. One point mutation (Gly353Ala) was identified. The Es-353G AChE and Es-353A AChE were expressed in baculovirus-insect cell system, respectively. The inhibition results showed that for eserine and Chlorpyrifos, Es-353A AChE was significantly less sensitive than Es-353G AChE. Meanwhile,comparison of the I50 values of eserine, dichlorvos, Chlorpyrifos and omethoate of recombinant Es AChEs with its host (Bombyx mori) AChEs indicated that, both Es AChEs are more sensitive than B. mori AChEs. The results give an insight of the mechanism that some OP insecticides can selectively kills Es while without distinct effect on its host, B. mori.
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참고문헌 (22)
참고문헌 신청
Aldridge, W. N. / 1972 / Enzyme inhibitors as substrates: interactions of esterases with esters of organophosphorus and carbamic acids / In Frontiers of Biology 26 : 41 ~ 44
Bourguet, D. / 1996 / Existence of two acetylcholinesterases in the mosquito Culex pipiens (Diptera: Culicidae) / J. Neurochem. 67 : 2115 ~ 2123
Chen, Z. / 2001 / The acetylcholinesterase gene and organophosphorus resistance in the Australian sheep blowfly, Lucilia cuprina / Insect Biochem Mol. Biol. 31 : 805 ~ 816
Ellman, G. L. / 1961 / A new and rapid colorimetric determination of acetylcholinesterase activity / Biochem. Pharmacol. 7 : 88 ~ 95
Fournier, D. / 1994 / Modification of AChE as a mechanism of resistance to insecticides / Comp. Biochem. Physiol. 108C : 19 ~ 31
Bourguet, D. / 1996 / Existence of two acetylcholinesterases in the mosquito Culex pipiens (Diptera: Culicidae) / J. Neurochem. 67 : 2115 ~ 2123
Chen, Z. / 2001 / The acetylcholinesterase gene and organophosphorus resistance in the Australian sheep blowfly, Lucilia cuprina / Insect Biochem Mol. Biol. 31 : 805 ~ 816
Ellman, G. L. / 1961 / A new and rapid colorimetric determination of acetylcholinesterase activity / Biochem. Pharmacol. 7 : 88 ~ 95
Fournier, D. / 1994 / Modification of AChE as a mechanism of resistance to insecticides / Comp. Biochem. Physiol. 108C : 19 ~ 31
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