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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 523 - 528 (6page)
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To identify novel genes that are regulated by promoter methylation,a combinational approach involving in silico mining followed by molecular assay was performed. From the expression microarray data registered in the European bioinformatics institute (EBI), genes showing downregulation in breast cancer cells were initially screened and then selected by e-Northern analysis using the Unigene database. A series of these in silico methods identified CAMK2B and ARFGEF1 as candidates, and the two genes were revealed to be hypermethylated in breast cancer cell lines and hypomethylated in normal breast cell lines. Additionally, cancer cell lines showed downregulated expression of these genes. Furthermore, treatment of the cancer cell lines with a demethylation agent, 5-Aza-2’-deoxycytidine,recovered expression of CAMK2B and ARFGEF1, implying that hypermethyaltion silenced gene activity in cancer cells. Taken together, promoter methylations of CAMK2B and ARFGEF1 are novel epigenetic markers identified in breast cancer cell lines and can be utilized for the application to clinical cancer tissues. [BMB reports 2011; 44(8): 523-528]
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참고문헌 (20)
참고문헌 신청
Ai, L / 2006 / Epigenetic silencing of the tumor suppressor cystatin M occurs during breast cancer progression / Cancer Res 66 : 7899 ~ 7909
Dworkin, A. M / 2009 / Epigenetic alterations in the breast: Implications for breast cancer detection, prognosis and treatment / Semin. Cancer Biol 19 : 165 ~ 171
Gaertner, T. R / 2004 / Comparative analyses of the three-dimensional structures and enzymatic properties of alpha, beta, gamma and delta isoforms of Ca2+-calmodulin-depende
Hesson, L / 2003 / NORE1A, a homologue of RASSF1A tumour suppressor gene is inactivated in human cancers / Oncogene 22 : 947 ~ 954
Ito, Y / 2008 / Somatically acquired hypomethylation of IGF2 in breast and colorectal cancer / Hum. Mol. Genet 17 : 2633 ~ 2643
Dworkin, A. M / 2009 / Epigenetic alterations in the breast: Implications for breast cancer detection, prognosis and treatment / Semin. Cancer Biol 19 : 165 ~ 171
Gaertner, T. R / 2004 / Comparative analyses of the three-dimensional structures and enzymatic properties of alpha, beta, gamma and delta isoforms of Ca2+-calmodulin-depende
Hesson, L / 2003 / NORE1A, a homologue of RASSF1A tumour suppressor gene is inactivated in human cancers / Oncogene 22 : 947 ~ 954
Ito, Y / 2008 / Somatically acquired hypomethylation of IGF2 in breast and colorectal cancer / Hum. Mol. Genet 17 : 2633 ~ 2643
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