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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 529 - 534 (6page)
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Ribosomal protein S3 (rpS3) is a multifunctional protein involved in translation, DNA repair, and apoptosis. The relationship between rpS3 and cyclin-dependent kinases (Cdks) involved in cell cycle regulation is not yet known. Here, we show that rpS3 is phosphorylated by Cdk1 in G2/M phase. Co-immunoprecipitation and GST pull-down assays revealed that Cdk1 interacted with rpS3. An in vitro kinase assay showed that Cdk1 phosphorylated rpS3 protein. Phosphorylation of rpS3 increased in nocodazole-arrested mitotic cells; however, treatment with Cdk1 inhibitor or Cdk1 siRNA significantly attenuated this phosphorylation event. The phosphorylation of a mutant form of rpS3, T221A, was significantly reduced compared with wild-type rpS3. Decreased phosphorylation and nuclear accumulation of T221A was much more pronounced in G2/M phase. These results suggest that the phosphorylation of rpS3 by Cdk1 occurs at Thr221 during G2/M phase and, moreover, that this event is important for nuclear accumulation of rpS3. [BMB reports 2011; 44(8): 529-534]
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참고문헌 (22)
참고문헌 신청
Bembenek, J / 2003 / Regulation of CDC14: pathways and checkpoints of mitotic exit / Front. Biosci 8 : d1275 ~ d1287
Bhat, K. P / 2004 / Essential role of ribosomal protein L11 in mediating growth inhibition-induced p53 activation / EMBO J 23 : 2402 ~ 2412
Chen, Y. C / 2007 / Mitochondrial ribosomal protein S36 delays cell cycle progression in association with p53 modification and p21(WAF1/CIP1) expression / J. Cell. Biochem 1
Dai, M. S / 2004 / Inhibition of MDM2-mediated p53 ubiquitination and degradation by ribosomal protein L5 / J. Biol. Chem 279 : 44475 ~ 44482
Francis, T. A / 1982 / Influence of the state of ribosome association on the phosphorylation of ribosomal proteins in isolated ribosome-protein kinase systems from rat cereb
Bhat, K. P / 2004 / Essential role of ribosomal protein L11 in mediating growth inhibition-induced p53 activation / EMBO J 23 : 2402 ~ 2412
Chen, Y. C / 2007 / Mitochondrial ribosomal protein S36 delays cell cycle progression in association with p53 modification and p21(WAF1/CIP1) expression / J. Cell. Biochem 1
Dai, M. S / 2004 / Inhibition of MDM2-mediated p53 ubiquitination and degradation by ribosomal protein L5 / J. Biol. Chem 279 : 44475 ~ 44482
Francis, T. A / 1982 / Influence of the state of ribosome association on the phosphorylation of ribosomal proteins in isolated ribosome-protein kinase systems from rat cereb
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