지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 602 - 607 (6page)
이용수
초록· 키워드
오류제보하기
Aberrant expression of microRNAs (miRNAs) plays important roles in carcinogenesis and tumor progression. However, the expression and biological role of miR-301b in triple-negative breast cancer (TNBC) remains unclear. Here we aimed to evaluate the roles and mechanisms of miR-301b in TNBC cells. miR-301b expression was assessed in TNBC specimens and cell lines by quantitative Real-Time PCR (qRT-PCR). TNBC cells were transfected with miR-301b mimics, inhibitors or Cylindromatosis (CYLD) small interfering RNA (siRNA) using Lipofectamine 2000. The functional roles of miR-301b were determined by cell proliferation, colony formation, and apoptosis assays. Western blots and qRT-PCR were used to measure the expression of mRNAs and proteins in the cells. We found that miR-301b was upregulated in TNBC specimens and cell lines. Overexpression of miR-301b promoted cell proliferation in TNBC cells, while inhibited the apoptosis induced by 5-FU. CYLD was downregulated by miR-301b at both mRNA and protein levels in TNBC cells. Dual-luciferase report assay confirmed that miR-301b downregulated CYLD by direct interaction with the 3’-untranslated region(3’-UTR) of CYLD mRNA. NF-B activation was mechanistically associated with miR-301b-mediated downregulation of CYLD. However, inhibition of miR-301b reversed all the effects of miR-301b. In conclusion, miR-301b plays an oncogenic role in TNBC possibly by downregulating CYLD and subsequently activating NF-B p65, and this may provide a novel therapeutic approach for TNBC.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
MiR-34a and miR-34b/c have distinct effects on the suppression of lung adenocarcinomas
Experimental and Molecular Medicine
2019 .01
-
MiR-34a, miR-21 and miR-23a as potential biomarkers for coronary artery disease: a pilot microarray study and confirmation in a 32 patient cohort
Experimental and Molecular Medicine
2015 .01
-
NMR hydrogen exchange study of miR156:miR156* duplexes
Journal of the Korean Magnetic Resonance Society
2019 .01
-
ANT2 shRNA downregulates miR-19a and miR-96 through the PI3K/Akt pathway and suppresses tumor growth in hepatocellular carcinoma cells
Experimental and Molecular Medicine
2016 .01
-
MicroRNA-375 is a therapeutic target for castration-resistant prostate cancer through the PTPN4/STAT3 axis
Experimental and Molecular Medicine
2022 .08
-
miR-30c regulates proliferation, apoptosis and differentiation via the Shh signaling pathway in P19 cells
Experimental and Molecular Medicine
2016 .01
-
miR-638 is a new biomarker for outcome prediction of non-small cell lung cancer patients receiving chemotherapy
Experimental and Molecular Medicine
2015 .01
-
MiR-371 promotes proliferation and metastasis in hepatocellular carcinoma by targeting PTEN
BMB Reports
2019 .01
-
microRNA-200a-3p increases 5-fluorouracil resistance by regulating dual specificity phosphatase 6 expression
Experimental and Molecular Medicine
2017 .01
-
MiR-125a-5p ameliorates monocrotalineinduced pulmonary arterial hypertension by targeting the TGF-β1 and IL-6/STAT3 signaling pathways
Experimental and Molecular Medicine
2018 .01
-
microRNA-25 as a novel modulator of circadian Period2 gene oscillation
Experimental and Molecular Medicine
2020 .09
-
MiR-449a attenuates autophagy of T-cell lymphoma cells by downregulating ATG4B expression
BMB Reports
2020 .01
-
MiR-155 modulates the inflammatory phenotype of intestinal myofibroblasts by targeting SOCS1 in ulcerative colitis
Experimental and Molecular Medicine
2015 .01
-
MicroRNA-22 negatively regulates LPS-induced inflammatory responses by targeting HDAC6 in macrophages
BMB Reports
2020 .01
-
K-MIR: 한국어 멀티모달 의도 인식 데이터셋
한국정보과학회 학술발표논문집
2024 .12
-
Prefrontal cortex miR-29b-3p plays a key role in the antidepressant-like effect of ketamine in rats
Experimental and Molecular Medicine
2018 .01
-
microRNA-328 in exosomes derived from M2 macrophages exerts a promotive effect on the progression of pulmonary fibrosis via FAM13A in a rat model
Experimental and Molecular Medicine
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0