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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 474 - 479 (6page)
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Three assay methods for quantification of the two galactoseoperon mRNAs that only differ by 5 bases in their 5’-end are presented. The 5’ ends of each mRNA were extended by ligating the 3’-end of the abundant 5S rRNA. This ligation extends the 5’ ends of the two gal mRNAs long enough to be distinguished by the specific PCR primers in the following quantification reactions. Quantification of the corresponding cDNAs was performed either by primer extension assay or real-time qPCR. To circumvent the problem of the RNA ligation reaction (i.e. very low ligation efficiency), we devised a new method that employs real-time qPCR directly for the quantification of the gal transcripts which differ by 5 bases in their 5’-ends.
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등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Adhya, S. / 1979 / Modulation of the two promoters of the galactose operon of Escherichia coli / Nature 279 : 492 ~ 494
Aki, T. / 1997 / Repressor induced site-specific binding of HU for transcriptional regulation / EMBO J. 16 : 3666 ~ 3674
Bingham, A. H. / 1986 / Mutations that reduce expression from the P2 promoter of the Escherichia coli galactose operon / Gene 41 : 67 ~ 74
Datsenko, K. A. / 2000 / One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products / Proc. Natl. Acad. Sci. U.S.A. : 6640 ~ 6645
Dreyfus, M. / 1985 / Point mutations that affect translation initiation in the Escherichia coli galE gene / J. Mol. Biol. 182 : 411 ~ 417
Aki, T. / 1997 / Repressor induced site-specific binding of HU for transcriptional regulation / EMBO J. 16 : 3666 ~ 3674
Bingham, A. H. / 1986 / Mutations that reduce expression from the P2 promoter of the Escherichia coli galactose operon / Gene 41 : 67 ~ 74
Datsenko, K. A. / 2000 / One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products / Proc. Natl. Acad. Sci. U.S.A. : 6640 ~ 6645
Dreyfus, M. / 1985 / Point mutations that affect translation initiation in the Escherichia coli galE gene / J. Mol. Biol. 182 : 411 ~ 417
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