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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 583 - 588 (6page)
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Microtubule actin crosslinking factor 1 (MACF1), a widely expressed cytoskeletal linker, plays important roles in various cells by regulating cytoskeleton dynamics. However, its role in osteoblastic cells is not well understood. Based on our previous findings that the association of MACF1 with F-actin and microtubules in osteoblast-like cells was altered under magnetic force conditions, here, by adopting a stable MACF1- knockdown MC3T3-E1 osteoblastic cell line, we found that MACF1 knockdown induced large cells with a binuclear/multinuclear structure. Further, immunofluorescence staining showed disorganization of F-actin and microtubules in MACF1-knockdown cells. Cell counting revealed significant decrease of cell proliferation and cell cycle analysis showed an S phase cell cycle arrest in MACF1-knockdown cells. Moreover and interestingly, MACF1 knockdown showed a potential effect on cellular MTT reduction activity and mitochondrial content, suggesting an impact on cellular metabolic activity. These results together indicate an important role of MACF1 in regulating osteoblastic cell morphology and function.
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참고문헌 (24)
참고문헌 신청
Al-Rubeai M / 1991 / Cell cycle, cell size and mitochondrial activity of hybridoma cells during batch cultivation / Cytotechnology 7 : 179 ~ 186
Antonellis PJ / 2014 / ACF7 is a hair-bundle antecedent, positioned to integrate cuticular plate actin and somatic tubulin / J Neurosci 34 : 305 ~ 312
Bernier G / 2000 / Acf7(MACF)is an actin and microtubule linker protein whose expression predominates in neural, muscle, and lung development / Dev Dyn 219 : 216 ~ 225
D'Avino PP / 2015 / Cytokinesis in animal cells / Cold Spring Harb Perspect Biol 7 : a015834 ~
Fuchs E / 2001 / Bridging cytoskeletal intersections / Genes Dev 15 : 1 ~ 14
Antonellis PJ / 2014 / ACF7 is a hair-bundle antecedent, positioned to integrate cuticular plate actin and somatic tubulin / J Neurosci 34 : 305 ~ 312
Bernier G / 2000 / Acf7(MACF)is an actin and microtubule linker protein whose expression predominates in neural, muscle, and lung development / Dev Dyn 219 : 216 ~ 225
D'Avino PP / 2015 / Cytokinesis in animal cells / Cold Spring Harb Perspect Biol 7 : a015834 ~
Fuchs E / 2001 / Bridging cytoskeletal intersections / Genes Dev 15 : 1 ~ 14
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