지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 25 - 30 (6page)
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초록· 키워드
오류제보하기
Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS,cell proliferation, cell cycle analyses, and p21Cip1 and p27Kip1expression levels were examined by bromodeoxyuridine assay,flow cytometry, real-time RT-PCR, and western blotting.
Immunohistochemical study noted a strong inverse correlation between Cx32 and Ki67 expression pattern as well as their location. In vitro, overexpression of Cx32 in AGS cells inhibited cell proliferation significantly. G1 arrest, up-regulation of cell cycle-regulatory proteins p21Cip1 and p27Kip1 was also found at both mRNA and protein levels. Taken together, Cx32 plays some roles in gastric cancer development by inhibiting gastric cancer cell proliferation through cell cycle arrest and cell cycle regulatory proteins.
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참고문헌 (33)
참고문헌 신청
Krutovskikh, V. A. / 2000 / Differential effect of subcellular localization of communication impairing gap junction protein connexin43 on tumor cell growth in vivo / Oncogene 19:505~513
Olbina, G. / 2003 / Mutations in the second extracellular region of connexin 43 prevent localization to the plasma membrane, but do not affect its ability to suppress cell growth / Mol. Cancer Res 1:690~700
Zhang, Y. W. / 2001 / Connexin43 suppresses proliferation of osteosarcoma U2OS cells through post-transcriptional regulation of p27 / Oncogene 20:4138~4149
Koffler, L. / 2000 / Growth inhibition in G(1) and altered expression of cyclin D1 and p27(kip-1) after forced connexin expression in lung and liver carcinoma cells / J. Cell. Biochem 79:347~354
Sanchez-Alvarez, R. / 2006 / Tolbutamide reduces glioma cell proliferation by increasing connexin43, which promotes the up-regulation of p21 and p27 and subsequent changes in retinoblastoma phosphorylation / Glia 54:125~134
Olbina, G. / 2003 / Mutations in the second extracellular region of connexin 43 prevent localization to the plasma membrane, but do not affect its ability to suppress cell growth / Mol. Cancer Res 1:690~700
Zhang, Y. W. / 2001 / Connexin43 suppresses proliferation of osteosarcoma U2OS cells through post-transcriptional regulation of p27 / Oncogene 20:4138~4149
Koffler, L. / 2000 / Growth inhibition in G(1) and altered expression of cyclin D1 and p27(kip-1) after forced connexin expression in lung and liver carcinoma cells / J. Cell. Biochem 79:347~354
Sanchez-Alvarez, R. / 2006 / Tolbutamide reduces glioma cell proliferation by increasing connexin43, which promotes the up-regulation of p21 and p27 and subsequent changes in retinoblastoma phosphorylation / Glia 54:125~134
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