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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 265 - 274 (10page)
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이 논문의 연구 히스토리 (2)
2008
Tau Class Glutathione S-transferases from Brassica rapa (BrGSTUs) : Genetic Mapping and Sequence Analysis
Tae-Ho Pa
,
k
,
Mina Jin
외 7명
한국원예학회 기타 간행물
2008.12
학술대회자료
Genetic mapping and sequence analysis of Phi class Glutathione S-transferases (BrGSTFs) candidates from Brassica rapa
Tae-Ho Pa
,
k
,
이상춘
외 9명
Journal of Plant Biotechnology
2008.01
학술저널
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초록· 키워드
오류제보하기
Glutathione S-transferases (GSTs) are multifunctional proteins encoded by a large gene family divided into Phi, Tau, Theta, Zeta, Lambda and DHAR classes on the basis of sequence identity. The Phi(F) and Tau(U) classes are plant-specific and ubiquitous. Their roles have been defined as herbicide detoxification and responses to biotic and abiotic stresses. Fifty-two members of the GST super-family were identified in the Arabidopsis thaliana genome, 13 members of which belong to the Phi class of GSTs (AtGSTFs). Based on the sequence similarities of AtGSTFs, 11 BAC clones were identified from Brassica rapa. Seven unique sequences of ORFs designated the Phi class candidates of GST derived from B. rapa (BrGSTFs) were detected from these 11 BAC clones by blast search and sequence alignment. Some of BrGSTFs were present in the same BAC clones indicating that BrGSTFs could also be clustered as usual in plant. They were mapped on B. rapa linkage group 2, 3, 9 and 10 and their nucleotide and amino acid sequences were highly similar to those of AtGSTFs. In addition, in silico analysis of BrGSTFs using Korea Brassica Genome Project 24K oligochip and microarray database for cold, salt and drought stresses revealed 15 unigenes to be highly similar to AtGSTFs and six of these were identical to one of BrGSTFs identified in the BAC clones indicating their expression. The sequences of BrGSTFs and unigenes identified in this study will facilitate further studies to apply GST genes to medical and agriculture purposes.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Ambrosone C. B. / 2004 / Breast cancer risk in premenopausal women is inversely associated with consumption of broccoli, a source of isothiocyanates, but is not modified by G
Buono D. D. / 2007 / Glutathione S-transferases in Festuca arundinacea: identification, characterization and inducible by safeners benoxacor / Phytochemistry 68 : 2614 ~ 2624
Burge C. B. / 1997 / Prediction of complete gene structure in human genomic DNA / J Mol Biol 268 : 78 ~ 94
Chen W. Q. / 1996 / The promoter of a H₂O₂-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites / Plant J 10 : 955 ~ 96
Cho H-Y / 2007 / Phi class glutathione S-transferase from Oryza sativa (OsGSTF5): molecular cloning, expression and biochemical characteristics / J Biochem Mol Biol 40 : 511
Buono D. D. / 2007 / Glutathione S-transferases in Festuca arundinacea: identification, characterization and inducible by safeners benoxacor / Phytochemistry 68 : 2614 ~ 2624
Burge C. B. / 1997 / Prediction of complete gene structure in human genomic DNA / J Mol Biol 268 : 78 ~ 94
Chen W. Q. / 1996 / The promoter of a H₂O₂-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites / Plant J 10 : 955 ~ 96
Cho H-Y / 2007 / Phi class glutathione S-transferase from Oryza sativa (OsGSTF5): molecular cloning, expression and biochemical characteristics / J Biochem Mol Biol 40 : 511
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