지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 203 - 209 (7page)
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초록· 키워드
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Genetic manipulation of pear (Pyrus pyrifolia Nakai) breeding is still difficult due to lack of reliable regeneration system. The aim of this research is to establish shoot regeneration system from leaf explants for pear (P. pyrifolia cv. Niitaka) using various concentrations of plant growth regulators and carbon source supplemented to medium. The highest regeneration rate of about 20% was found on a medium containing 4.4 g/L of Murashige and Skoog (MS) without vitamins, Linsmaier and Skoog (LS)vitamins were added separately. Leaf explants of pear were cultured on MS medium containing 7 g/L of Daishin agar supplemented with various concentrations of NAA (0.01,0.05, 0.1, 0.5 mg/L) in combination with BA(3, 5, 10 mg/L)for shoot regeneration. In medium with 5 mg/L of BA and 0.01 mg/L of NAA, adventitious shoot regeneration rate was higher than others treated. The optimal results were observed using MS medium supplemented with 30 g/L sorbitol as carbon source on regeneration system. Sorbitol is considered better carbon source than sucrose for shoot regeneration of pear (P. pyrifolia cv. Niitaka). In order to increase of shoot regeneration in pear (P. pyrifolia cv. Niitaka), plant agar and Daishin agar used as gelling agents, Daishin agar is more efficient in shoot regeneration.
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등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Rubtsov GA / 1944 / Geographical distribution of the genus Pyrus and trends and factors in its evolution / Am Nat 78:358~366
Seo MS / 2002 / Investigation of transformation efficiency of rice using Agrobacterium tumefaciens and high transformation of GPAT (glycerol-3-phosphate acyltransferase) gene relative to chilling tolerance / Korean J Plant Biotechnology 29:85~92
Singha S / 1984 / Influence of two commercial agars on in vitro shoot proliferation of ‘Almey’ crabapple and Pyrus communis ‘Seckel’ / Hort Science 19:227~228
Sotiropoulos TE / 2006 / Sucrose and sorbitol effects on shoot growth and proliferation in vitro nutritional status and peroxidase and catalase isoenzymes of M9 and MM106 apple (Malus domestica Borkh.) rootstocks / Europ J Hort Sci 71:114~119
Tukey HB / 1934 / Artificial culture methods for isolated embryos of deciduous fruits / Proc Am Soc Hort Sci 32:630~665
Seo MS / 2002 / Investigation of transformation efficiency of rice using Agrobacterium tumefaciens and high transformation of GPAT (glycerol-3-phosphate acyltransferase) gene relative to chilling tolerance / Korean J Plant Biotechnology 29:85~92
Singha S / 1984 / Influence of two commercial agars on in vitro shoot proliferation of ‘Almey’ crabapple and Pyrus communis ‘Seckel’ / Hort Science 19:227~228
Sotiropoulos TE / 2006 / Sucrose and sorbitol effects on shoot growth and proliferation in vitro nutritional status and peroxidase and catalase isoenzymes of M9 and MM106 apple (Malus domestica Borkh.) rootstocks / Europ J Hort Sci 71:114~119
Tukey HB / 1934 / Artificial culture methods for isolated embryos of deciduous fruits / Proc Am Soc Hort Sci 32:630~665
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