지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 210 - 216 (7page)
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초록· 키워드
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Efficient regeneration methods and transformation system are a priority for successful application of genetic engineering to vegetative propagated plants such as grape (Vitis labrusca L.). This research is to establish shoot regeneration system from plant explants for ‘Campbell Early’, ‘Tamnara’, ‘Heukgoosul’, ‘Heukbosek’ using two types of plant growth regulators supplemented to medium. The highest adventitious shoot regeneration rate of 5% was achieved on a medium containing of Murashige and Skoog (MS) inorganic salts and Linsmaier and Skoog (LS) vitamins, 2 mg/L of TDZ and 0.1 mg/L of IBA. Leaf tissue of ‘Campbell Early’, was co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, gus gene as reporter gene and resistance to kanamycin as selective agent, the other Agrobacterium strains, GV3101 containing the vector pB7 WG2D carrying with mPAP1-D gene. mPAP1-D is a regulatory genes of the anthocyanin biosynthetic pathway. ‘Campbell Early’ harboring mPAP1-D gene was readily able to be selected by red color due to anthocyanin accumulation in the transformed shoot. These results might be helpful for further studies to enhance the transformation efficiency in grape.
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참고문헌 (24)
참고문헌 신청
Belarmino MM / 2000 / Agrobacterium-mediated genetic transformation of a phalenopsis orchid / Plant Cell Rep 19 : 435 ~ 442
Burrow MD / 1990 / High frequency generation of transgenic tobacco plants after modified leaf disk cocultivation with Agrobacterium tumefaciens / Plant Mol Biol Rep 8 : 124 ~
Chee R / 1987 / Improved inorganic media constituents for in vitro shoot multiplication of Vitis / Scientia Hort 32 : 85 ~ 95
Dhekney SA / 2009 / Factorsinfluencing genetic transformation and plant regeneration of Vitis / Am J Enol Vitic 60 : 285 ~ 292
Goussard PG / 1982 / Morphological responses of shoot apices of grapevine cultured in vitro effects of cytokinins in routine subculturing / Vitis 21 : 293 ~ 298
Burrow MD / 1990 / High frequency generation of transgenic tobacco plants after modified leaf disk cocultivation with Agrobacterium tumefaciens / Plant Mol Biol Rep 8 : 124 ~
Chee R / 1987 / Improved inorganic media constituents for in vitro shoot multiplication of Vitis / Scientia Hort 32 : 85 ~ 95
Dhekney SA / 2009 / Factorsinfluencing genetic transformation and plant regeneration of Vitis / Am J Enol Vitic 60 : 285 ~ 292
Goussard PG / 1982 / Morphological responses of shoot apices of grapevine cultured in vitro effects of cytokinins in routine subculturing / Vitis 21 : 293 ~ 298
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