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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 127 - 132 (6page)
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In bioreactor cultures of plants, inoculum density is an important factor affecting growth and proliferation of the plantlets. To maximize shoot growth and proliferation of grape rootstock '5BB' in bioreactors, inoculum density varied at 15, 30, 45 and 60 single nodes in a 3-liter scale balloon type bioreactor, respectively and cultured for 40 days. Results suggested that the growth and the photosynthesis of the plantlet were greatly affected by inoculum density in the bioreactor. The inoculum density of 45 nodes resulted in the greatest growth (910.4 mg/shoot FW, 764.4 mg/root FW) followed by 30 nodes. CO2 assimilation rate, stomatal conductance, transpiration rate of the plantlet were also highest at the inoculum density of 45 nodes. Significant reduces in shoot and root growth (426.5 mg/shoot FW, 248.4 mg/root FW) were observed at the inoculum density of 60 nodes. When the inoculum density decreased by 15 nodes, plantlets were malformed due to hyperhydricity, resulting in the highest transpiration rate and the lowest CO2 assimilation rate. The plantlets stressed by the inoculum density at 15 nodes and 60 nodes showed larger number and irregular shape of stomata compared to the plantlets inoculated with 45 nodes.
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참고문헌 (21)
참고문헌 신청
Akalezi C. O. / 1999 / Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin producton by suspension culture of Panax ginseng
Barlass M. / 1978 / In vitro propagation of grape (Vitis vinifera) from fragmented shoot apiece / Vitis 117 : 335 ~ 340
Drew A. P. / 1992 / Acclimatizing micro propagated black cherry by comparison with halfsib seedlings / Plant Physiol 86 : 459 ~ 464
Hahn E. J. / 2003 / Proliferation of multiple shoots and microbulbs of garlic (Allium sativumL.) through bioreactor system / Proceedings of the 1st International Congress on Biorea
Hahn E. J. / 2005 / Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems / Plant Cell Tiss Org Cul
Barlass M. / 1978 / In vitro propagation of grape (Vitis vinifera) from fragmented shoot apiece / Vitis 117 : 335 ~ 340
Drew A. P. / 1992 / Acclimatizing micro propagated black cherry by comparison with halfsib seedlings / Plant Physiol 86 : 459 ~ 464
Hahn E. J. / 2003 / Proliferation of multiple shoots and microbulbs of garlic (Allium sativumL.) through bioreactor system / Proceedings of the 1st International Congress on Biorea
Hahn E. J. / 2005 / Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems / Plant Cell Tiss Org Cul
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