지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 330 - 334 (5page)
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초록· 키워드
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To investigate optimal conditions for plant regeneration in Platycodon grandiflorum (Jacq. A. DC.).Both leaf and hypocotyl explants were cultured on Murashige& Skoog’s (MS) medium supplemented with combinations of 0.1, 0.5, 1.0, or 2.0 mg/L cytokinins (BA and kinetin) and 1.0 mg/L 2,4-D for 6 weeks, respectively. According to the type of explant, the total shoot organogenesis (56.38%) in leaf explants was higher than in hypocotyls (28.20%). In comparison with kinetin and BA for the plant regeneration, the frequency (70.38%) of leaf explants was higher in combination with kinetin and 2,4-D than of BA with 2,4-D (42.38%), whereas the frequency (35.56%) of hypocotyls explants was higher in BA combination than kinetin combination (20.83%). Thehighest frequency (94.20%) was observed from the cultures of leaf explants on the MS medium supplemented with 1.0 mg/L kinetin and 1.0 mg/L 2,4-D. Upon transfer onto 1 /2 MS basal medium containing 3% sucrose, shoots developed into plantlets with roots, and were well grown in soil in the greenhouse.
These results lead us to speculate that the optimization of culture conditions was responsible for the mass propagation from in vitro cultures of Platycodon grandiflorum (Jacq. A.
DC.).
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Barwale UB / 1986 / Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node / Theor. Appl. Genet 72 : 423 ~ 428
Cho DY / 1998 / Plant regeneration from somatic embryo with structural diversity from leaf explants cultures of Osteicum koreanum Kitagawa / Kor J Plant Tiss Cult 25 : 51 ~ 5
Cho HJ / 2002 / Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus / Plant Cell, Tiss Org Cult 69 (3) : 259 ~ 269
Chung JH / 2002 / Tissue cultures of Platycodon grandiflour / J Agricult Life Sci 36 : 9 ~ 18
Erisen S / 2010 / Callus induction and plant regeneration of endemic Astragalus nezaketae in Turkey / Electron of Biotechnol
Cho DY / 1998 / Plant regeneration from somatic embryo with structural diversity from leaf explants cultures of Osteicum koreanum Kitagawa / Kor J Plant Tiss Cult 25 : 51 ~ 5
Cho HJ / 2002 / Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus / Plant Cell, Tiss Org Cult 69 (3) : 259 ~ 269
Chung JH / 2002 / Tissue cultures of Platycodon grandiflour / J Agricult Life Sci 36 : 9 ~ 18
Erisen S / 2010 / Callus induction and plant regeneration of endemic Astragalus nezaketae in Turkey / Electron of Biotechnol
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